Detailed information of TA system TA089532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelB(antitoxin)
• Location: 263105..263717
• Replicon: chromosome
• Accession: NZ_CP025029
• Organism: Streptococcus agalactiae strain SGEHI2015-25

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8G2N8L3
• Locus tag: CWQ20_RS01640
• Protein ID: WP_000384860.1
• Coordinates: 263105..263440 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q8E7D2
• Locus tag: CWQ20_RS01645
• Protein ID: WP_000259017.1
• Coordinates: 263430..263717 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWQ20_RS01610	258303..258944	+	642	WP_000591144.1	hypothetical protein	-
CWQ20_RS01620	259244..260119	+	876	WP_000421240.1	hypothetical protein	-
CWQ20_RS01625	260155..260589	+	435	WP_100831568.1	hypothetical protein	-
CWQ20_RS01630	260906..262162	+	1257	WP_000122836.1	plasmid recombination protein	-
CWQ20_RS01635	262330..262800	+	471	WP_000130119.1	hypothetical protein	-
CWQ20_RS01640	263105..263440	-	336	WP_000384860.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWQ20_RS01645	263430..263717	-	288	WP_000259017.1	hypothetical protein	Antitoxin
CWQ20_RS01650	264224..264514	+	291	WP_000078283.1	WXG100 family type VII secretion target	-
CWQ20_RS01655	264616..264984	+	369	WP_001882138.1	hypothetical protein	-
CWQ20_RS01660	265022..265582	+	561	WP_001865562.1	hypothetical protein	-
CWQ20_RS01665	265555..266235	+	681	WP_001865565.1	hypothetical protein	-
CWQ20_RS01670	266220..266606	+	387	WP_000259069.1	hypothetical protein	-
CWQ20_RS01675	266640..266921	+	282	WP_017647916.1	hypothetical protein	-
CWQ20_RS01695	267764..268624	+	861	WP_000477654.1	Rgg/GadR/MutR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	255136..263991	8855

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13227.87 Da
• Isoelectric Point: 4.5348

>T89532 WP_000384860.1 NZ_CP025029:c263440-263105 [Streptococcus agalactiae]
MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIINDIEKLEVFPEVGFDADEKYGSEISNYHSTRGYTLSKD
YIVLYHIEEEENSVVIDYLLPTRSDYMKLFK

Nucleotide

• Length: 336 bp

>T89532 NZ_CP025029:c263440-263105 [Streptococcus agalactiae]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCCCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAATGACATAGAAAAACTTGAAGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAGAAATCAGCAATTATCACAGTACTAGAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAAGAGGAAGAGAATAGCGTTGTCATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10906.50 Da
• Isoelectric Point: 4.7271

>AT89532 WP_000259017.1 NZ_CP025029:c263717-263430 [Streptococcus agalactiae]
MVTVEKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKEKLFKQFQAEISKNIEDVRQ
GKFYTSEEVRAELGL

Nucleotide

• Length: 288 bp

>AT89532 NZ_CP025029:c263717-263430 [Streptococcus agalactiae]
ATGGTTACAGTAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCCGAAATCAGCAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Antitoxin

• AlphaFold DB: A0A076Z4V6