Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 174343..174998 Replicon plasmid pRLN3
Accession NZ_CP025015
Organism Rhizobium leguminosarum strain Norway

Toxin (Protein)


Gene name vapC Uniprot ID A0A432N9U1
Locus tag CUJ84_RS33100 Protein ID WP_018496488.1
Coordinates 174343..174762 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7W6UNS6
Locus tag CUJ84_RS33105 Protein ID WP_018496487.1
Coordinates 174762..174998 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUJ84_RS33080 170982..172256 - 1275 WP_105010009.1 plasmid partitioning protein RepA -
CUJ84_RS33090 172774..173878 + 1105 Protein_137 IS5 family transposase -
CUJ84_RS33095 173875..174219 + 345 WP_105009780.1 hypothetical protein -
CUJ84_RS33100 174343..174762 - 420 WP_018496488.1 type II toxin-antitoxin system VapC family toxin Toxin
CUJ84_RS33105 174762..174998 - 237 WP_018496487.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CUJ84_RS33115 176131..177198 + 1068 WP_105009782.1 TlpA family protein disulfide reductase -
CUJ84_RS33125 177674..178876 + 1203 WP_105009783.1 cytochrome P450 -
CUJ84_RS38130 179439..179844 + 406 Protein_143 cytochrome P450 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..557386 557386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-117)

Antitoxin

SpoVT-AbrB domain

(7-44)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15616.86 Da        Isoelectric Point: 4.9496

>T89514 WP_018496488.1 NZ_CP025015:c174762-174343 [Rhizobium leguminosarum]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPARELDNLTALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALDTVLVTNNTRDFLAYPGLKLENWLDAERRETN

Download         Length: 420 bp

>T89514 NZ_CP025015:c174762-174343 [Rhizobium leguminosarum]
ATGGCGCGCTACATGCTCGATACGAACATGTGTATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCTCGCCGCTTTGC
CAGTTGCTATGTGGGGGACGTGGTGATGTCCGCCATCACCTTCGCGGAACTCGAGTATGGTGTTTCCGCATCCGATGATC
CGGCACGAGAACTTGATAATCTCACCGCCCTGGCCGAGCTGATCACGGTAGAGCCGTTTGGGATCGCAGCAGCGCGTGCC
TACGGTCCGGTTCGAATGGCGACGAGAGACCGGAAGAAAGATCATCTCGACAAACTGATCGCATCGCATGCGATCGCTCT
GGACACGGTATTGGTGACCAACAACACGCGGGACTTCCTGGCGTATCCCGGACTGAAGCTCGAAAACTGGCTGGACGCGG
AACGCAGAGAAACCAATTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9051.15 Da        Isoelectric Point: 5.6454

>AT89514 WP_018496487.1 NZ_CP025015:c174998-174762 [Rhizobium leguminosarum]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQDERDKI

Download         Length: 237 bp

>AT89514 NZ_CP025015:c174998-174762 [Rhizobium leguminosarum]
ATGCACACCACTAAAGCTTTCAAAAATGGAAATTCCCAGGCGGTTCGCATACCGGCGGAACTGGCGTTTGAGCGAACCGA
TGTTGAGTTGGAGATCGAGCGCATCGGCGACGAGCTTCGCATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTCATGCGTGACGGAAGAGAGTCCCATGAGCAAGACGAGCGGGATAAGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A432N9U1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6UNS6

References