Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 126277..127049 Replicon plasmid pRLN2
Accession NZ_CP025014
Organism Rhizobium leguminosarum strain Norway

Toxin (Protein)


Gene name tacT Uniprot ID A0A2K9ZEQ1
Locus tag CUJ84_RS30110 Protein ID WP_018069827.1
Coordinates 126561..127049 (+) Length 163 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A2K9ZEL5
Locus tag CUJ84_RS30105 Protein ID WP_026170739.1
Coordinates 126277..126561 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUJ84_RS30070 121474..122070 - 597 WP_105009298.1 SOS response-associated peptidase -
CUJ84_RS30075 122100..122930 - 831 WP_105009299.1 Ku protein -
CUJ84_RS30080 122940..123829 - 890 Protein_110 Ku protein -
CUJ84_RS30085 123903..124157 + 255 WP_105009300.1 hypothetical protein -
CUJ84_RS30090 124574..124887 + 314 Protein_112 EamA family transporter -
CUJ84_RS30095 125036..125785 + 750 WP_105009301.1 sulfite exporter TauE/SafE family protein -
CUJ84_RS30100 125907..126055 + 149 Protein_114 transcriptional regulator -
CUJ84_RS30105 126277..126561 + 285 WP_026170739.1 DUF1778 domain-containing protein Antitoxin
CUJ84_RS30110 126561..127049 + 489 WP_018069827.1 GNAT family N-acetyltransferase Toxin
CUJ84_RS30115 127314..127652 - 339 WP_105009650.1 helix-turn-helix domain-containing protein -
CUJ84_RS38090 128072..128284 - 213 WP_156925179.1 hypothetical protein -
CUJ84_RS30125 128565..128825 + 261 WP_018496974.1 DUF982 domain-containing protein -
CUJ84_RS30135 129134..129358 - 225 WP_105009303.1 hypothetical protein -
CUJ84_RS38450 129828..130205 + 378 WP_199788869.1 hypothetical protein -
CUJ84_RS30150 130529..131572 + 1044 WP_105009305.1 non-homologous end-joining DNA ligase -
CUJ84_RS30155 131586..131885 - 300 WP_105009306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..592529 592529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-142)

Antitoxin

Vibrio phage ICP1, Orf50

(15-86)


Sequences

Toxin        


Download         Length: 163 a.a.        Molecular weight: 17277.88 Da        Isoelectric Point: 7.9271

>T89513 WP_018069827.1 NZ_CP025014:126561-127049 [Rhizobium leguminosarum]
MLSAPTLLGEAHDLQLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEGERVVGYYCLSSGALAVADAPGAIRRNMPDPV
PMAVLGRLAIDRNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHFGFAASPANPMALVLSLKAQVG
LK

Download         Length: 489 bp

>T89513 NZ_CP025014:126561-127049 [Rhizobium leguminosarum]
ATGTTGTCGGCACCGACGCTTCTTGGCGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGACAGCCTTGATGAATG
GCTGCGCCGCAGGGCGCGGGCCAATCAGGTCAGCGGCGCATCGCGAACATATGTGGTCTGCGAGGGTGAGCGCGTTGTCG
GTTATTACTGCCTCTCATCGGGAGCGCTCGCGGTCGCGGATGCGCCGGGAGCGATCCGGCGCAACATGCCCGACCCGGTT
CCCATGGCAGTCCTTGGCCGTCTGGCTATCGATCGCAACTGGCAGGGAAAAGGAATCGGAGCCGCGCTGTTGCAAGACGC
CGTGCTGCGTACCGGACAGGCAGCCCATATCATGGGTATTCGCGGCCTTCTGGTTCACGCAATCTCCGATGAAGCCAAGG
CCTTCTATGAGCATTTTGGATTTGCAGCGTCGCCCGCGAATCCTATGGCCTTGGTGTTGTCATTGAAAGCTCAAGTAGGC
CTGAAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10660.02 Da        Isoelectric Point: 8.6086

>AT89513 WP_026170739.1 NZ_CP025014:126277-126561 [Rhizobium leguminosarum]
MTSKNATDTHTRAVNLRVREDVRVLIDRAAKAHGKTRSDFMIDASRRAAEDALLDQTLVRVDPDSYKHYLAVLDQPPSSE
GFERLMNAPKPWQP

Download         Length: 285 bp

>AT89513 NZ_CP025014:126277-126561 [Rhizobium leguminosarum]
ATGACCAGCAAAAACGCCACTGACACCCATACCCGCGCCGTTAACTTGAGGGTTCGTGAGGACGTTCGCGTCCTGATCGA
CCGCGCCGCCAAGGCCCACGGGAAAACCCGTTCGGATTTTATGATCGACGCTTCGCGCCGTGCCGCCGAAGACGCGCTTC
TCGATCAGACATTGGTGCGGGTCGATCCTGACAGCTACAAACACTATCTCGCCGTCCTCGACCAGCCGCCAAGTAGCGAA
GGCTTCGAACGGTTGATGAACGCTCCCAAACCCTGGCAGCCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K9ZEQ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2K9ZEL5

References