Detailed information of TA system TA089512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 671145..671644
• Replicon: plasmid pRLN1
• Accession: NZ_CP025013
• Organism: Rhizobium leguminosarum strain Norway

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CUJ84_RS27590
• Protein ID: WP_105008817.1
• Coordinates: 671384..671644 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: CUJ84_RS27585
• Protein ID: WP_105008816.1
• Coordinates: 671145..671387 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ84_RS27565	666912..668543	+	1632	WP_105008812.1	ABC transporter ATP-binding protein	-
CUJ84_RS27570	668566..669786	+	1221	WP_105008813.1	M20/M25/M40 family metallo-hydrolase	-
CUJ84_RS38420	669789..669956	+	168	Protein_630	aminopeptidase P family N-terminal domain-containing protein	-
CUJ84_RS27580	670079..670579	+	501	WP_105008815.1	ankyrin repeat domain-containing protein	-
CUJ84_RS27585	671145..671387	+	243	WP_105008816.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CUJ84_RS27590	671384..671644	+	261	WP_105008817.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUJ84_RS27600	671966..673537	-	1572	WP_105008818.1	amidohydrolase family protein	-
CUJ84_RS27605	673534..674583	-	1050	WP_105008819.1	DUF4243 domain-containing protein	-
CUJ84_RS27610	674625..675062	-	438	WP_105008820.1	nucleoside deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	virB11	1..1098158	1098158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9788.21 Da
• Isoelectric Point: 4.5069

>T89512 WP_105008817.1 NZ_CP025013:671384-671644 [Rhizobium leguminosarum]
MSFKLSAEAEEDIIAIAEQGVRVFGAGQAKRYHDELFALFDLIAVNPCIARERDEIDPPVRIHPFKAHLVVYRIEDDETI
FVQPCC

Nucleotide

• Length: 261 bp

>T89512 NZ_CP025013:671384-671644 [Rhizobium leguminosarum]
ATGAGTTTTAAGCTTTCTGCCGAAGCGGAAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTGTGTTCGGGGCAGG
CCAGGCAAAGCGATACCACGATGAACTTTTCGCGTTATTTGATCTGATAGCTGTCAATCCATGCATCGCGCGCGAACGGG
ATGAGATTGATCCGCCTGTTAGAATTCATCCCTTCAAAGCGCATCTAGTCGTTTATCGGATCGAAGATGATGAGACAATT
TTTGTACAACCCTGCTGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.95 Da
• Isoelectric Point: 6.2805

>AT89512 WP_105008816.1 NZ_CP025013:671145-671387 [Rhizobium leguminosarum]
MATMNVSLPGPMKDWVEAQARTGRYSNASDYVRDLIRRDQTRNDKIAAMQSFVEAGLQSDVGIQSKDELFAEAIARASKS

Nucleotide

• Length: 243 bp

>AT89512 NZ_CP025013:671145-671387 [Rhizobium leguminosarum]
ATGGCCACGATGAACGTATCTCTTCCAGGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAGGACCGGGCGATATTCTAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAGACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGATGTCGGCATCCAATCAAAGGATGAACTCTTTGCTGAGGCGATAGCTCGTGCCAGCAAGTCA
TGA