Detailed information of TA system TA089511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 445719..446317
• Replicon: plasmid pRLN1
• Accession: NZ_CP025013
• Organism: Rhizobium leguminosarum strain Norway

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CUJ84_RS26395
• Protein ID: WP_081929559.1
• Coordinates: 446024..446317 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2A6K8H2
• Locus tag: CUJ84_RS26390
• Protein ID: WP_062578764.1
• Coordinates: 445719..446027 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ84_RS26370	441190..442272	+	1083	WP_105008639.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
CUJ84_RS26375	442269..443027	+	759	WP_105008640.1	sugar phosphate isomerase/epimerase	-
CUJ84_RS26380	443107..444159	+	1053	WP_105008641.1	sugar phosphate isomerase/epimerase	-
CUJ84_RS26385	444354..445541	+	1188	WP_105008642.1	Gfo/Idh/MocA family oxidoreductase	-
CUJ84_RS26390	445719..446027	+	309	WP_062578764.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CUJ84_RS26395	446024..446317	+	294	WP_081929559.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CUJ84_RS26400	446391..446999	-	609	WP_105008643.1	J domain-containing protein	-
CUJ84_RS26405	447160..447339	-	180	WP_105008644.1	hypothetical protein	-
CUJ84_RS26410	447491..447946	-	456	WP_105008645.1	GNAT family N-acetyltransferase	-
CUJ84_RS26420	448470..448850	+	381	WP_105008646.1	RidA family protein	-
CUJ84_RS26425	448984..449787	-	804	WP_105008647.1	helix-turn-helix transcriptional regulator	-
CUJ84_RS26430	449991..451058	+	1068	WP_105009176.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	virB11	1..1098158	1098158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10651.22 Da
• Isoelectric Point: 7.0106

>T89511 WP_081929559.1 NZ_CP025013:446024-446317 [Rhizobium leguminosarum]
VKLTWSAFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLVDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTR

Nucleotide

• Length: 294 bp

>T89511 NZ_CP025013:446024-446317 [Rhizobium leguminosarum]
GTGAAGCTTACCTGGTCTGCGTTCGCGTTATCGGATCGCGACGCCATTTTCACATACATAGAAGCAGAAAATCCGTCAGC
CGCCATACTGGTCGACGAACGGATCGTTGCTGCAGTCCGTAGGTTAGTAGATTTTCCCGCGAGCGGTCGTGTTGGCAGAA
TTGCTGGCACCCGCGAACTGGTAATTAACGGCACGCCGTACGTCGCGGCGTATGCAATTACCGAAACAGCGGTTCGTATT
CTTCGCGTCCTCCATGGTGCGCAGGAATGGCCAGACACTTTGCCAACGCGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10998.32 Da
• Isoelectric Point: 5.1570

>AT89511 WP_062578764.1 NZ_CP025013:445719-446027 [Rhizobium leguminosarum]
MAANALVQTRIDAEVRDRASAVLGNMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDVSDD
EVEAHFAERRAAARLNAGARKS

Nucleotide

• Length: 309 bp

>AT89511 NZ_CP025013:445719-446027 [Rhizobium leguminosarum]
ATGGCTGCAAATGCACTGGTACAAACTCGTATCGATGCCGAGGTTCGGGATCGTGCCTCCGCAGTGCTTGGAAATATGGG
GCTGACCGTATCTGATGCGGTTCGTATCTTACTCACCCGAACGGCCAACGAAGGTGCGCTGCCGCTGGAGCTTCTTTCCG
GCAGCGAGGCCCATGACGCTTGGTTTCGCACCAAGGTACTTGAAGCGCTGAACGATACTCGACCGGACGTCTCAGATGAT
GAAGTCGAGGCGCATTTCGCTGAACGTCGAGCGGCAGCCCGCCTTAACGCGGGTGCGCGCAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A6K8H2