Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 248132..248808 Replicon plasmid pRLN1
Accession NZ_CP025013
Organism Rhizobium leguminosarum strain Norway

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CUJ84_RS25450 Protein ID WP_105008487.1
Coordinates 248132..248557 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag CUJ84_RS25455 Protein ID WP_105009160.1
Coordinates 248554..248808 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUJ84_RS25430 243394..244050 - 657 WP_105008483.1 sigma-70 family RNA polymerase sigma factor -
CUJ84_RS25435 244214..244705 + 492 WP_105008484.1 GNAT family N-acetyltransferase -
CUJ84_RS25440 244826..245608 - 783 WP_105008485.1 siderophore-iron reductase FhuF -
CUJ84_RS25445 245618..247798 - 2181 WP_105008486.1 TonB-dependent siderophore receptor -
CUJ84_RS25450 248132..248557 - 426 WP_105008487.1 type II toxin-antitoxin system VapC family toxin Toxin
CUJ84_RS25455 248554..248808 - 255 WP_105009160.1 plasmid stabilization protein Antitoxin
CUJ84_RS25460 249116..249688 - 573 WP_105008488.1 TetR/AcrR family transcriptional regulator -
CUJ84_RS25465 249797..250483 + 687 WP_105008489.1 glutathione S-transferase -
CUJ84_RS25470 250580..251551 + 972 WP_105008490.1 alpha/beta hydrolase -
CUJ84_RS25475 251625..252029 - 405 WP_105008491.1 hypothetical protein -
CUJ84_RS25480 252230..252655 + 426 WP_105008492.1 MarR family winged helix-turn-helix transcriptional regulator -
CUJ84_RS25485 252723..253616 + 894 WP_105008493.1 NAD(P)H-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - virB11 1..1098158 1098158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15315.62 Da        Isoelectric Point: 5.1351

>T89510 WP_105008487.1 NZ_CP025013:c248557-248132 [Rhizobium leguminosarum]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPRGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYTELMARARAAGRAIGKADAYIAAIAAANGFIVATRDSSPFEAAGLNVINPWYAPT

Download         Length: 426 bp

>T89510 NZ_CP025013:c248557-248132 [Rhizobium leguminosarum]
ATGATCCTGATCGATACCAACGTCATCTCGGAGCCATGGAAGCCCACACCCGATAATCAGGTGCTGGCGTGGATCGATGC
CCAAGCAGTGGAAACGCTGTTCGTTTCAGCGATTACAGTAGCTGAATTACGGTTCGGCATTGCCGCGATGCCTAGAGGAA
GGCGAAAGACAGTCCTCTACGATCGACTAGAACAGGAAGTTCTGCCTCTATTTTCAGGACGCGTTCTATCCTTCGACATG
GCTGCGTCACAGAGCTATACGGAATTAATGGCGCGAGCACGCGCAGCAGGTCGGGCAATCGGTAAAGCAGATGCCTATAT
TGCCGCAATTGCCGCGGCGAACGGATTTATTGTCGCTACCCGCGACTCTAGTCCCTTCGAAGCCGCAGGACTTAACGTTA
TCAACCCTTGGTACGCACCAACATAG

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9262.47 Da        Isoelectric Point: 6.2235

>AT89510 WP_105009160.1 NZ_CP025013:c248808-248554 [Rhizobium leguminosarum]
MAAVTIRNLSEETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKTPAEP
MSFE

Download         Length: 255 bp

>AT89510 NZ_CP025013:c248808-248554 [Rhizobium leguminosarum]
ATGGCAGCAGTTACAATACGCAATTTGTCTGAGGAGACACATCGTGCCTTGAGAGTGAGGGCAGCTCATCACGGCCGCAG
TACCGAAGCAGAAATCCGTGACATCCTAGAAACAGCGGTACGGCCAGCTGAGCGCGTGATGTTGGGCTCGTTGCTAGCAT
CAATCGGACGCGAGGCAGGGCTTTCCAACAACGACGTGGATAATCTGCAGCTCGTCCGAGACAAAACGCCTGCGGAACCT
ATGAGTTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References