Detailed information of TA system TA089509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 52615..53306
• Replicon: plasmid pRLN1
• Accession: NZ_CP025013
• Organism: Rhizobium leguminosarum strain Norway

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CUJ84_RS24550
• Protein ID: WP_105008335.1
• Coordinates: 52615..53043 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CUJ84_RS24555
• Protein ID: WP_105008336.1
• Coordinates: 53040..53306 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ84_RS24525	49059..49189	+	131	Protein_45	TetR family transcriptional regulator	-
CUJ84_RS24530	49460..50299	+	840	WP_105008333.1	transporter substrate-binding domain-containing protein	-
CUJ84_RS24535	50463..51125	+	663	WP_047622998.1	amino acid ABC transporter permease	-
CUJ84_RS24540	51132..51791	+	660	WP_024319797.1	amino acid ABC transporter permease	-
CUJ84_RS24545	51804..52574	+	771	WP_105008334.1	amino acid ABC transporter ATP-binding protein	-
CUJ84_RS24550	52615..53043	-	429	WP_105008335.1	type II toxin-antitoxin system VapC family toxin	Toxin
CUJ84_RS24555	53040..53306	-	267	WP_105008336.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CUJ84_RS24560	53566..54315	+	750	WP_105008337.1	GntR family transcriptional regulator	-
CUJ84_RS24565	54319..55104	+	786	WP_105008338.1	transporter substrate-binding domain-containing protein	-
CUJ84_RS24570	55185..55853	+	669	WP_105008339.1	amino acid ABC transporter permease	-
CUJ84_RS24575	55853..56512	+	660	WP_105008340.1	amino acid ABC transporter permease	-
CUJ84_RS24580	56490..57215	+	726	WP_003562518.1	amino acid ABC transporter ATP-binding protein	-
CUJ84_RS24585	57290..57593	-	304	Protein_57	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	virB11	1..1098158	1098158
	-	flank	IS/Tn	-	-	57845..59218	1373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-69)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15779.06 Da
• Isoelectric Point: 4.6772

>T89509 WP_105008335.1 NZ_CP025013:c53043-52615 [Rhizobium leguminosarum]
VRLLLDTNVLSEVTRPRPDARVLEWLDGLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVAIAWGDLMGLAKRSGHGLSSMDSLIAATAIAHDLTLATRNIKDFEGLGIELVDPWAERP

Nucleotide

• Length: 429 bp

>T89509 NZ_CP025013:c53043-52615 [Rhizobium leguminosarum]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGACCGCGTCCCGATGCACGTGTCCTAGAATGGCTGGA
TGGGCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCACTGATGGAAAGCG
GTCGGAAGCGCGACGCGCTGGCCGAATGGCTTGCCCAGGATCTGCCGCAGCGCTTCGAGCAGCGCGTCATCCCTGTGGAC
CAGCCGGTTGCGATAGCCTGGGGCGACCTGATGGGGCTCGCAAAGCGAAGCGGACACGGCTTATCGTCAATGGACAGCTT
GATTGCCGCCACCGCGATTGCCCACGATCTCACCCTCGCCACCCGCAATATAAAGGATTTCGAGGGCTTGGGGATCGAGC
TTGTCGACCCGTGGGCCGAGCGCCCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10006.43 Da
• Isoelectric Point: 10.4689

>AT89509 WP_105008336.1 NZ_CP025013:c53306-53040 [Rhizobium leguminosarum]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADMDLERRR
DEPRDLQL

Nucleotide

• Length: 267 bp

>AT89509 NZ_CP025013:c53306-53040 [Rhizobium leguminosarum]
ATGCAGCCATCACGACCAGAGGATAAAAGTTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACACCGCAAACGATCATGCGCAATGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CGGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCCCTGCGTGGCGCCGATATGGATCTCGAACGTCGACGC
GACGAACCGCGTGATCTCCAGCTGTGA