Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17499..18142 Replicon plasmid pAUSMDU8119-2
Accession NZ_CP025010
Organism Klebsiella pneumoniae strain AUSMDU00008119

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CWN89_RS28860 Protein ID WP_001044770.1
Coordinates 17726..18142 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CWN89_RS28855 Protein ID WP_001261282.1
Coordinates 17499..17729 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWN89_RS28810 12697..13170 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
CWN89_RS28815 13262..13492 + 231 WP_011977773.1 hypothetical protein -
CWN89_RS28820 14384..15166 - 783 WP_004152340.1 site-specific integrase -
CWN89_RS28825 15166..15498 - 333 WP_004152339.1 hypothetical protein -
CWN89_RS28830 15505..15903 - 399 WP_004171440.1 hypothetical protein -
CWN89_RS28835 15929..16258 - 330 WP_004152337.1 hypothetical protein -
CWN89_RS28840 16286..16594 - 309 WP_004152336.1 hypothetical protein -
CWN89_RS30355 17081..17542 - 462 WP_072093212.1 hypothetical protein -
CWN89_RS28855 17499..17729 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CWN89_RS28860 17726..18142 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CWN89_RS28865 18216..18926 + 711 WP_004152334.1 ATP-binding protein -
CWN89_RS28870 18960..19657 + 698 Protein_20 IS1 family transposase -
CWN89_RS28875 19673..19783 + 111 Protein_21 mercuric transport protein periplasmic component -
CWN89_RS28880 19819..20241 + 423 WP_001340589.1 organomercurial transporter MerC -
CWN89_RS28885 20293..21987 + 1695 WP_000105636.1 mercury(II) reductase -
CWN89_RS28890 22005..22367 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
CWN89_RS28895 22364..22600 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1A / blaOXA-9 / aac(6')-Ib / blaKPC-2 - 1..118202 118202
- flank IS/Tn - - 11309..12577 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T89507 WP_001044770.1 NZ_CP025010:17726-18142 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T89507 NZ_CP025010:17726-18142 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT89507 WP_001261282.1 NZ_CP025010:17499-17729 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT89507 NZ_CP025010:17499-17729 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References