Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 17499..18142 | Replicon | plasmid pAUSMDU8119-2 |
| Accession | NZ_CP025010 | ||
| Organism | Klebsiella pneumoniae strain AUSMDU00008119 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | CWN89_RS28860 | Protein ID | WP_001044770.1 |
| Coordinates | 17726..18142 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | CWN89_RS28855 | Protein ID | WP_001261282.1 |
| Coordinates | 17499..17729 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CWN89_RS28810 | 12697..13170 | + | 474 | WP_004152341.1 | YkgJ family cysteine cluster protein | - |
| CWN89_RS28815 | 13262..13492 | + | 231 | WP_011977773.1 | hypothetical protein | - |
| CWN89_RS28820 | 14384..15166 | - | 783 | WP_004152340.1 | site-specific integrase | - |
| CWN89_RS28825 | 15166..15498 | - | 333 | WP_004152339.1 | hypothetical protein | - |
| CWN89_RS28830 | 15505..15903 | - | 399 | WP_004171440.1 | hypothetical protein | - |
| CWN89_RS28835 | 15929..16258 | - | 330 | WP_004152337.1 | hypothetical protein | - |
| CWN89_RS28840 | 16286..16594 | - | 309 | WP_004152336.1 | hypothetical protein | - |
| CWN89_RS30355 | 17081..17542 | - | 462 | WP_072093212.1 | hypothetical protein | - |
| CWN89_RS28855 | 17499..17729 | + | 231 | WP_001261282.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| CWN89_RS28860 | 17726..18142 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| CWN89_RS28865 | 18216..18926 | + | 711 | WP_004152334.1 | ATP-binding protein | - |
| CWN89_RS28870 | 18960..19657 | + | 698 | Protein_20 | IS1 family transposase | - |
| CWN89_RS28875 | 19673..19783 | + | 111 | Protein_21 | mercuric transport protein periplasmic component | - |
| CWN89_RS28880 | 19819..20241 | + | 423 | WP_001340589.1 | organomercurial transporter MerC | - |
| CWN89_RS28885 | 20293..21987 | + | 1695 | WP_000105636.1 | mercury(II) reductase | - |
| CWN89_RS28890 | 22005..22367 | + | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
| CWN89_RS28895 | 22364..22600 | + | 237 | WP_000993386.1 | broad-spectrum mercury transporter MerE | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1A / blaOXA-9 / aac(6')-Ib / blaKPC-2 | - | 1..118202 | 118202 | |
| - | flank | IS/Tn | - | - | 11309..12577 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T89507 WP_001044770.1 NZ_CP025010:17726-18142 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T89507 NZ_CP025010:17726-18142 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT89507 WP_001261282.1 NZ_CP025010:17499-17729 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT89507 NZ_CP025010:17499-17729 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |