Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53717..54453 Replicon plasmid pAUSMDU8119-1
Accession NZ_CP025009
Organism Klebsiella pneumoniae strain AUSMDU00008119

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CWN89_RS27975 Protein ID WP_003026803.1
Coordinates 53971..54453 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CWN89_RS27970 Protein ID WP_003026799.1
Coordinates 53717..53983 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWN89_RS27925 49779..50141 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CWN89_RS27930 50191..50541 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CWN89_RS27935 50899..51168 + 270 WP_004152102.1 hypothetical protein -
CWN89_RS27940 51156..51731 + 576 WP_004152103.1 hypothetical protein -
CWN89_RS27945 51762..52256 + 495 WP_004152104.1 DNA-binding protein -
CWN89_RS27950 52300..52668 + 369 WP_004152105.1 hypothetical protein -
CWN89_RS27955 52702..52905 + 204 WP_004152106.1 hemolysin expression modulator Hha -
CWN89_RS27960 52954..53211 + 258 WP_004152107.1 hypothetical protein -
CWN89_RS27965 53287..53541 + 255 WP_004152108.1 hypothetical protein -
CWN89_RS27970 53717..53983 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CWN89_RS27975 53971..54453 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CWN89_RS27980 54665..56011 + 1347 WP_020314316.1 ISNCY family transposase -
CWN89_RS27985 56512..57766 - 1255 Protein_57 IS3 family transposase -
CWN89_RS27990 57854..58816 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / aac(6')-Ib / blaKPC-2 - 1..176049 176049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T89506 WP_003026803.1 NZ_CP025009:53971-54453 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T89506 NZ_CP025009:53971-54453 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89506 WP_003026799.1 NZ_CP025009:53717-53983 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89506 NZ_CP025009:53717-53983 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References