Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53715..54451 Replicon plasmid pAUSMDU3562-1
Accession NZ_CP025006
Organism Klebsiella pneumoniae strain AUSMDU00003562

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CWN90_RS27945 Protein ID WP_003026803.1
Coordinates 53969..54451 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CWN90_RS27940 Protein ID WP_003026799.1
Coordinates 53715..53981 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWN90_RS27895 49777..50139 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CWN90_RS27900 50189..50539 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CWN90_RS27905 50897..51166 + 270 WP_004152102.1 hypothetical protein -
CWN90_RS27910 51154..51729 + 576 WP_004152103.1 hypothetical protein -
CWN90_RS27915 51760..52254 + 495 WP_004152104.1 DNA-binding protein -
CWN90_RS27920 52298..52666 + 369 WP_004152105.1 hypothetical protein -
CWN90_RS27925 52700..52903 + 204 WP_004152106.1 hemolysin expression modulator Hha -
CWN90_RS27930 52952..53209 + 258 WP_004152107.1 hypothetical protein -
CWN90_RS27935 53285..53539 + 255 WP_004152108.1 hypothetical protein -
CWN90_RS27940 53715..53981 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CWN90_RS27945 53969..54451 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CWN90_RS27950 54663..56009 + 1347 WP_020314316.1 ISNCY family transposase -
CWN90_RS27955 56510..57764 - 1255 Protein_58 IS3 family transposase -
CWN90_RS27960 57852..58814 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..167373 167373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T89487 WP_003026803.1 NZ_CP025006:53969-54451 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T89487 NZ_CP025006:53969-54451 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89487 WP_003026799.1 NZ_CP025006:53715-53981 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89487 NZ_CP025006:53715-53981 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References