Detailed information of TA system TA089468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 5021094..5022682
• Replicon: chromosome
• Accession: NZ_CP025003
• Organism: Dickeya fangzhongdai strain DSM 101947

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: CVE23_RS22510
• Protein ID: WP_100850374.1
• Coordinates: 5021363..5022682 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2K8QTG6
• Locus tag: CVE23_RS22505
• Protein ID: WP_038669098.1
• Coordinates: 5021094..5021363 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVE23_RS22480	5016416..5017027	-	612	WP_100850371.1	NAD(P)-dependent oxidoreductase	-
CVE23_RS22485	5017169..5017549	+	381	WP_100850372.1	helix-turn-helix transcriptional regulator	-
CVE23_RS22490	5017650..5018735	-	1086	WP_038920707.1	4-hydroxyphenylpyruvate dioxygenase	-
CVE23_RS22495	5019051..5019824	+	774	WP_100850373.1	MBL fold metallo-hydrolase	-
CVE23_RS22500	5019868..5020824	+	957	WP_208622096.1	helix-turn-helix domain-containing protein	-
CVE23_RS22505	5021094..5021363	+	270	WP_038669098.1	helix-turn-helix domain-containing protein	Antitoxin
CVE23_RS22510	5021363..5022682	+	1320	WP_100850374.1	type II toxin-antitoxin system HipA family toxin	Toxin
CVE23_RS22515	5022717..5024081	-	1365	WP_100850375.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
CVE23_RS22520	5024197..5025828	-	1632	WP_100850376.1	membrane protein insertase YidC	-
CVE23_RS22525	5025831..5026088	-	258	WP_013320102.1	membrane protein insertion efficiency factor YidD	-
CVE23_RS22530	5026052..5026411	-	360	WP_013320101.1	ribonuclease P protein component	-
CVE23_RS22535	5026430..5026570	-	141	WP_004093983.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49234.37 Da
• Isoelectric Point: 6.5751

>T89468 WP_100850374.1 NZ_CP025003:5021363-5022682 [Dickeya fangzhongdai]
MAALDVYMNGYRVGTLTKTGSGAHHFSYDENWLVLPGSRPISLSMPLRHQPYQGNEVYNFFDNLLPDNPDIRRRIVARHN
ADSTQPFDLLAKVGQDSVGALQLVPQGTPVGDIKQIEYKTLSEQQLENILAGYLADAPLGMIDAEDDFRISIAGAQEKTA
LLYLGDHWCLPFNTTPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCMLVAKAFGLQVPHCFMITAGKIKALAVERFDRK
YASDGRWIMRLPQEDFCQVLNVPSARKYENHGGPGISAIMSYLLGAVDPEKDRYSFMKTQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAQAVGFARESMESILTEFAQSM
DAVVVNVRNQLPADFPAPIRDAILEGMQARARRLMTGWE

Nucleotide

• Length: 1320 bp

>T89468 NZ_CP025003:5021363-5022682 [Dickeya fangzhongdai]
ATGGCTGCGCTCGATGTTTACATGAACGGCTATCGAGTGGGGACGTTGACCAAAACGGGTAGCGGCGCTCATCATTTTTC
CTATGATGAGAACTGGCTTGTGTTGCCGGGGAGCCGCCCCATCTCGCTCTCGATGCCGCTACGCCATCAGCCCTATCAAG
GTAATGAAGTTTATAATTTCTTCGACAATTTATTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGACATAAC
GCAGATTCTACCCAACCGTTTGACCTGCTGGCTAAAGTGGGGCAGGACAGTGTCGGGGCGTTGCAATTGGTGCCGCAGGG
TACACCTGTTGGCGATATAAAACAGATCGAATACAAAACTTTATCCGAACAACAGCTTGAAAACATACTGGCGGGATACC
TGGCCGATGCGCCACTGGGGATGATTGATGCAGAAGATGATTTTCGTATCTCTATTGCTGGTGCACAGGAAAAGACGGCG
CTGCTCTATCTGGGGGATCACTGGTGTTTGCCCTTCAATACCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCATTCTTACTCAATTGATATGTCTGACAGCGTTGAGAATGAGTATCTGTGCATGCTCGTGGCCAAGGCGT
TTGGGCTGCAGGTACCGCACTGTTTTATGATCACAGCAGGCAAGATAAAAGCGCTGGCGGTAGAACGTTTTGATCGTAAA
TACGCTTCGGATGGTCGTTGGATTATGCGGTTGCCGCAGGAGGATTTTTGCCAGGTATTAAACGTTCCTTCCGCACGGAA
ATACGAAAATCACGGTGGGCCGGGTATTTCCGCCATTATGTCGTATCTATTGGGTGCGGTTGATCCAGAAAAGGATCGTT
ATAGCTTCATGAAGACTCAGGTGCTGTTTTGGCTATTAGCCGCAACAGATGGGCATGCGAAAAATTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGTCTCACGCCTTTCTATGACATTCTCTCGATGTACCCGGCGATGGGCGGGCGGGGAATTGA
CCGTAGAGAGGCCAAACTGGCGATGGGATTGACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTCCCACGCC
ACTTTTTCCAAACGGCGCAGGCAGTTGGATTTGCCAGAGAATCGATGGAGAGTATTCTGACGGAGTTTGCGCAATCGATG
GATGCTGTTGTGGTGAACGTCAGAAATCAGCTGCCTGCGGATTTTCCTGCACCTATTCGTGATGCGATTCTCGAAGGTAT
GCAGGCCAGGGCAAGGCGATTAATGACCGGGTGGGAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9915.17 Da
• Isoelectric Point: 10.0982

>AT89468 WP_038669098.1 NZ_CP025003:5021094-5021363 [Dickeya fangzhongdai]
MKVTNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTITPRNANAANNEASA
ATSSWKEEW

Nucleotide

• Length: 270 bp

>AT89468 NZ_CP025003:5021094-5021363 [Dickeya fangzhongdai]
ATGAAGGTCACCAACAGCAAACAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGAAAAGT
AGCCAGTAAAGTGGGTATTCGCCAGGACACGGTATCCAGCTTCGAACAACACCCTGATTCCACCAAGCTGGAAACATTCT
TTAAAATCCTGTCGGCATTGAATTTAGAACTTACGATTACTCCCAGAAACGCAAATGCCGCCAATAACGAGGCTTCTGCT
GCAACATCATCCTGGAAGGAAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8QTG6