Detailed information of TA system TA089459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1864660..1865277
• Replicon: chromosome
• Accession: NZ_CP025003
• Organism: Dickeya fangzhongdai strain DSM 101947

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2K8QKH7
• Locus tag: CVE23_RS08470
• Protein ID: WP_071842437.1
• Coordinates: 1865074..1865277 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CVE23_RS08465
• Protein ID: WP_100849304.1
• Coordinates: 1864660..1865070 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVE23_RS08440	1860135..1861052	+	918	WP_100849301.1	iron-siderophore ABC transporter substrate-binding protein	-
CVE23_RS08445	1861049..1862071	+	1023	WP_100849302.1	iron ABC transporter permease	-
CVE23_RS08450	1862082..1863113	+	1032	WP_039695444.1	iron ABC transporter permease	-
CVE23_RS08455	1863125..1863934	+	810	WP_049854931.1	ABC transporter ATP-binding protein	-
CVE23_RS08460	1863967..1864584	+	618	WP_100849303.1	RraA family protein	-
CVE23_RS08465	1864660..1865070	-	411	WP_100849304.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CVE23_RS08470	1865074..1865277	-	204	WP_071842437.1	type II toxin-antitoxin system HicA family toxin	Toxin
CVE23_RS08475	1865598..1865957	+	360	WP_100849305.1	hypothetical protein	-
CVE23_RS08480	1866262..1867317	-	1056	WP_100849306.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7347.47 Da
• Isoelectric Point: 11.0566

>T89459 WP_071842437.1 NZ_CP025003:c1865277-1865074 [Dickeya fangzhongdai]
MDSRTLIAEIKADGWELVRVNGSHHHFTHPTKPGLVTIPHPKKDLPIGTVKSIRKQAGISSSLTQRG

Nucleotide

• Length: 204 bp

>T89459 NZ_CP025003:c1865277-1865074 [Dickeya fangzhongdai]
ATGGATAGCAGGACGTTGATAGCAGAAATCAAGGCCGATGGATGGGAGCTGGTAAGGGTTAACGGCAGTCATCACCATTT
TACCCACCCAACCAAACCAGGATTAGTGACCATCCCACACCCTAAGAAGGATTTACCGATAGGCACGGTAAAAAGCATCA
GAAAACAAGCGGGGATATCATCCTCACTGACTCAAAGAGGCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14571.59 Da
• Isoelectric Point: 4.3494

>AT89459 WP_100849304.1 NZ_CP025003:c1865070-1864660 [Dickeya fangzhongdai]
MFYPIAIETGDDTHAYGVTVPDLPGCFSAGDTLDEAIANAKEAITGHIELLIEMGQDIPAVSSVGVLAKTPEYTGYTWAV
VDIDVTRLMGGAEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSIR

Nucleotide

• Length: 411 bp

>AT89459 NZ_CP025003:c1865070-1864660 [Dickeya fangzhongdai]
ATGTTTTATCCAATTGCCATTGAAACAGGCGACGATACTCATGCATACGGCGTGACAGTGCCCGATCTGCCGGGCTGTTT
CTCCGCAGGTGATACACTTGATGAGGCTATCGCCAATGCTAAAGAAGCGATCACCGGCCACATAGAGCTGCTGATCGAGA
TGGGCCAGGATATTCCCGCCGTGTCATCCGTAGGTGTGCTGGCGAAAACACCGGAGTACACCGGTTACACCTGGGCTGTT
GTTGATATCGATGTCACTCGGCTGATGGGAGGGGCGGAAAAAATCAACGTCACACTGCCGAAGTCGCTGATTGACCGTAT
AGACCGCTGCGTAGCGAGTAACCCGGAATTTAAGAGCCGCTCAGGATTTCTTGCTCAGGCAGCGTTAGAGCGGATTTCAT
CCATTCGCTAA

Structures

Toxin

• AlphaFold DB: A0A2K8QKH7