Detailed information of TA system TA089453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 470733..471475
• Replicon: chromosome
• Accession: NZ_CP025003
• Organism: Dickeya fangzhongdai strain DSM 101947

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: C6C3X4
• Locus tag: CVE23_RS02210
• Protein ID: WP_015855199.1
• Coordinates: 470733..471212 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A3N0G0N1
• Locus tag: CVE23_RS02215
• Protein ID: WP_019843650.1
• Coordinates: 471203..471475 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVE23_RS02180	466580..466780	+	201	WP_188726100.1	AlpA family transcriptional regulator	-
CVE23_RS02185	466777..467316	+	540	WP_100848772.1	ash family protein	-
CVE23_RS02190	467309..469351	+	2043	WP_100848773.1	AAA family ATPase	-
CVE23_RS02195	469574..469840	-	267	WP_013316114.1	helix-turn-helix domain-containing protein	-
CVE23_RS02200	469946..470158	+	213	Protein_418	transcriptional regulator	-
CVE23_RS02205	470303..470704	-	402	WP_100848774.1	hypothetical protein	-
CVE23_RS02210	470733..471212	-	480	WP_015855199.1	GNAT family N-acetyltransferase	Toxin
CVE23_RS02215	471203..471475	-	273	WP_019843650.1	DUF1778 domain-containing protein	Antitoxin
CVE23_RS02220	471741..472859	+	1119	Protein_422	IS3 family transposase	-
CVE23_RS02225	473122..473844	-	723	WP_100848775.1	methyltransferase domain-containing protein	-
CVE23_RS02230	473852..474109	-	258	WP_049855262.1	YjhX family toxin	-
CVE23_RS02235	474562..474900	-	339	WP_100848776.1	hypothetical protein	-
CVE23_RS22595	475288..475986	-	699	WP_145958393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	460787..480654	19867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17538.30 Da
• Isoelectric Point: 9.5367

>T89453 WP_015855199.1 NZ_CP025003:c471212-470733 [Dickeya fangzhongdai]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVASEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T89453 NZ_CP025003:c471212-470733 [Dickeya fangzhongdai]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACACTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGTAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTACTTGGCCGATTAGCCGTTGATGAACGTTATCAACGCATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCGCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10388.86 Da
• Isoelectric Point: 4.6702

>AT89453 WP_019843650.1 NZ_CP025003:c471475-471203 [Dickeya fangzhongdai]
MRTETKEMPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRVN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT89453 NZ_CP025003:c471475-471203 [Dickeya fangzhongdai]
ATGCGAACAGAGACAAAAGAAATGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCTGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAACGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGTTGGAATCACCTGTCACAGATAATCCGCGTGTGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A2K8QHE3

Antitoxin

• AlphaFold DB: A0A3N0G0N1