Detailed information of TA system TA089451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3791935..3792572
• Replicon: chromosome
• Accession: NZ_CP025001
• Organism: Bacillus siamensis strain SCSIO 05746

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CWD84_RS19530
• Protein ID: WP_003156187.1
• Coordinates: 3791935..3792285 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CWD84_RS19535
• Protein ID: WP_003156188.1
• Coordinates: 3792291..3792572 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWD84_RS19490	3786972..3787574	-	603	WP_060963542.1	SpoIIE family protein phosphatase	-
CWD84_RS19495	3787574..3788362	-	789	WP_060963543.1	RNA polymerase sigma factor SigB	-
CWD84_RS19500	3788328..3788810	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
CWD84_RS19505	3788807..3789136	-	330	WP_016937169.1	anti-sigma factor antagonist	-
CWD84_RS19510	3789200..3790207	-	1008	WP_016937170.1	PP2C family protein-serine/threonine phosphatase	-
CWD84_RS19515	3790219..3790620	-	402	WP_003156178.1	anti-sigma regulatory factor	-
CWD84_RS19520	3790623..3790988	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CWD84_RS19525	3790993..3791814	-	822	WP_060963544.1	STAS domain-containing protein	-
CWD84_RS19530	3791935..3792285	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CWD84_RS19535	3792291..3792572	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CWD84_RS19540	3792692..3793861	-	1170	WP_016937171.1	alanine racemase	-
CWD84_RS19545	3793977..3794984	-	1008	WP_016937172.1	outer membrane lipoprotein carrier protein LolA	-
CWD84_RS19550	3795149..3795514	-	366	WP_060963545.1	holo-ACP synthase	-
CWD84_RS19555	3795607..3796206	+	600	WP_016937174.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T89451 WP_003156187.1 NZ_CP025001:c3792285-3791935 [Bacillus siamensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T89451 NZ_CP025001:c3792285-3791935 [Bacillus siamensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT89451 WP_003156188.1 NZ_CP025001:c3792572-3792291 [Bacillus siamensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT89451 NZ_CP025001:c3792572-3792291 [Bacillus siamensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCACAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAACATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5