Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 2972380..2973302 | Replicon | chromosome |
| Accession | NZ_CP025001 | ||
| Organism | Bacillus siamensis strain SCSIO 05746 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | A0A120KSF0 |
| Locus tag | CWD84_RS14915 | Protein ID | WP_060962999.1 |
| Coordinates | 2972380..2973132 (+) | Length | 251 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | I2HQ14 |
| Locus tag | CWD84_RS14920 | Protein ID | WP_003154807.1 |
| Coordinates | 2973132..2973302 (+) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CWD84_RS14895 | 2967770..2968720 | - | 951 | WP_060962997.1 | ring-cleaving dioxygenase | - |
| CWD84_RS14900 | 2969043..2970359 | + | 1317 | WP_060962998.1 | amino acid permease | - |
| CWD84_RS14905 | 2970644..2971261 | + | 618 | WP_032874614.1 | DUF47 domain-containing protein | - |
| CWD84_RS14910 | 2971274..2972272 | + | 999 | WP_044802872.1 | inorganic phosphate transporter | - |
| CWD84_RS14915 | 2972380..2973132 | + | 753 | WP_060962999.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| CWD84_RS14920 | 2973132..2973302 | + | 171 | WP_003154807.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| CWD84_RS22125 | 2973399..2973524 | + | 126 | WP_141228459.1 | hypothetical protein | - |
| CWD84_RS14925 | 2973561..2974439 | - | 879 | WP_060963000.1 | N-acetylmuramoyl-L-alanine amidase | - |
| CWD84_RS14930 | 2974453..2974716 | - | 264 | WP_016936430.1 | phage holin | - |
| CWD84_RS14935 | 2974730..2974993 | - | 264 | WP_003154815.1 | hemolysin XhlA family protein | - |
| CWD84_RS14940 | 2975045..2975845 | - | 801 | WP_060965050.1 | hypothetical protein | - |
| CWD84_RS14945 | 2975897..2976061 | - | 165 | WP_060963001.1 | XkdX family protein | - |
| CWD84_RS14950 | 2976061..2976393 | - | 333 | WP_060963002.1 | XkdW family protein | - |
| CWD84_RS14955 | 2976414..2978147 | - | 1734 | WP_060963003.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2964224..3008865 | 44641 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 251 a.a. Molecular weight: 29357.82 Da Isoelectric Point: 4.5756
>T89450 WP_060962999.1 NZ_CP025001:2972380-2973132 [Bacillus siamensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFSAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSETLLTEFDYLLFTSLTSIYDLML
PIEEEEEGDG
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFSAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSETLLTEFDYLLFTSLTSIYDLML
PIEEEEEGDG
Download Length: 753 bp
>T89450 NZ_CP025001:2972380-2973132 [Bacillus siamensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCCGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTTCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCTCTTATTGATGCGTTT
ATCTTTTTGAGCGCATATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGACACGAGGGAGATTTTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCGGCACAGTCGGATAAGGACCGATTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGAAAAGACGTCTACTATGATCAAAAGGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTATGTGATCA
AACTGACGTCAGAAACCCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTCACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGAGGAAGGTGACGGATAA
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCCGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTTCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCTCTTATTGATGCGTTT
ATCTTTTTGAGCGCATATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGACACGAGGGAGATTTTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCGGCACAGTCGGATAAGGACCGATTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGAAAAGACGTCTACTATGATCAAAAGGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTATGTGATCA
AACTGACGTCAGAAACCCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTCACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGAGGAAGGTGACGGATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6676.74 Da Isoelectric Point: 10.9273
>AT89450 WP_003154807.1 NZ_CP025001:2973132-2973302 [Bacillus siamensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
Download Length: 171 bp
>AT89450 NZ_CP025001:2973132-2973302 [Bacillus siamensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAAGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAACGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAAGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAACGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A120KSF0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I2HQ14 |