Detailed information of TA system TA089423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3320822..3321515
• Replicon: chromosome
• Accession: NZ_CP024996
• Organism: Herbaspirillum rubrisubalbicans strain DSM 11543

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: RC54_RS14870
• Protein ID: WP_061789518.1
• Coordinates: 3320822..3321199 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0U3CND7
• Locus tag: RC54_RS14875
• Protein ID: WP_017450286.1
• Coordinates: 3321180..3321515 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC54_RS14845	3316980..3317648	+	669	WP_061789520.1	GntR family transcriptional regulator	-
RC54_RS14850	3317674..3318573	-	900	WP_017450291.1	pseudouridine synthase	-
RC54_RS14855	3318570..3319907	-	1338	WP_061789519.1	type II toxin-antitoxin system HipA family toxin	-
RC54_RS14860	3319904..3320206	-	303	WP_058895877.1	helix-turn-helix domain-containing protein	-
RC54_RS14865	3320427..3320615	-	189	WP_058895878.1	YjfB family protein	-
RC54_RS14870	3320822..3321199	+	378	WP_061789518.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RC54_RS14875	3321180..3321515	+	336	WP_017450286.1	helix-turn-helix domain-containing protein	Antitoxin
RC54_RS14880	3321594..3321782	-	189	WP_017450285.1	hypothetical protein	-
RC54_RS14885	3321971..3322813	-	843	WP_061789517.1	anti-sigma factor	-
RC54_RS14890	3322810..3323367	-	558	WP_058895881.1	RNA polymerase sigma factor	-
RC54_RS14895	3323385..3323756	-	372	WP_017450282.1	hypothetical protein	-
RC54_RS14900	3323951..3326380	-	2430	WP_061789516.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-96)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13975.16 Da
• Isoelectric Point: 9.8096

>T89423 WP_061789518.1 NZ_CP024996:3320822-3321199 [Herbaspirillum rubrisubalbicans]
MFTNEKPLEWIASSYKDLMALPAEVRQRFGFALSLAQMGERDDTAKVLKGFGGAGVLEVVEYQMGNAYRAVSTVKFEEAV
FVLHCFQKKSKSGIATPHTEMDIVRPRLNVAAARAEELKHAKKNR

Nucleotide

• Length: 378 bp

>T89423 NZ_CP024996:3320822-3321199 [Herbaspirillum rubrisubalbicans]
ATGTTTACCAATGAGAAGCCGCTCGAGTGGATTGCGAGCAGCTACAAGGATCTGATGGCGCTGCCTGCGGAGGTTCGTCA
GCGTTTCGGCTTTGCCCTGTCGCTTGCCCAGATGGGAGAGCGCGACGATACGGCCAAGGTCTTGAAAGGTTTCGGCGGTG
CCGGGGTATTGGAAGTTGTCGAATACCAAATGGGCAATGCCTACCGCGCGGTCTCCACGGTGAAATTCGAAGAAGCGGTG
TTCGTGCTGCATTGCTTTCAGAAAAAGAGCAAAAGCGGCATCGCCACTCCCCACACGGAGATGGACATCGTTCGGCCACG
TCTCAACGTGGCCGCAGCAAGAGCCGAGGAATTGAAACATGCAAAAAAGAATCGTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12286.51 Da
• Isoelectric Point: 10.4695

>AT89423 WP_017450286.1 NZ_CP024996:3321180-3321515 [Herbaspirillum rubrisubalbicans]
MQKRIVEETEVLRSTSNVFADLGLPQADKLRIKTGLVIEIRKAMRALGLTQQAAAQRMGIPQPKVSGMMRGDFSNLSERK
LMDCLNRLGYDIDIRVCPATATVGQLKLTVV

Nucleotide

• Length: 336 bp

>AT89423 NZ_CP024996:3321180-3321515 [Herbaspirillum rubrisubalbicans]
ATGCAAAAAAGAATCGTTGAAGAAACCGAAGTCCTTCGCAGCACCAGCAACGTCTTCGCGGACCTGGGATTGCCACAGGC
CGACAAGCTGCGCATCAAGACCGGGCTGGTGATCGAAATCCGCAAGGCCATGCGCGCCCTTGGACTTACCCAGCAAGCGG
CGGCACAGCGCATGGGGATTCCGCAACCGAAGGTGTCGGGCATGATGCGCGGTGACTTCAGCAATCTTTCCGAGCGCAAG
TTAATGGATTGTCTGAATCGACTGGGTTATGACATCGATATCCGGGTGTGCCCCGCTACCGCTACGGTAGGACAATTGAA
GCTCACGGTCGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0U3CND7