Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4856653..4857291 Replicon chromosome
Accession NZ_CP024978
Organism Escherichia coli strain CV839-15

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CV83915_RS28690 Protein ID WP_000813794.1
Coordinates 4857115..4857291 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CV83915_RS28685 Protein ID WP_001270286.1
Coordinates 4856653..4857069 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83915_RS28665 4851805..4852746 - 942 WP_001251313.1 ABC transporter permease -
CV83915_RS28670 4852747..4853760 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
CV83915_RS28675 4853778..4854923 - 1146 WP_063079343.1 ABC transporter substrate-binding protein -
CV83915_RS28680 4855168..4856574 - 1407 WP_063079344.1 PLP-dependent aminotransferase family protein -
CV83915_RS28685 4856653..4857069 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CV83915_RS28690 4857115..4857291 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CV83915_RS28695 4857513..4857743 + 231 WP_074150579.1 DUF2554 family protein -
CV83915_RS28700 4857835..4859796 - 1962 WP_001619413.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CV83915_RS28705 4859869..4860405 - 537 WP_016244590.1 DNA-binding transcriptional regulator SutR -
CV83915_RS28710 4860458..4861672 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4861712..4862860 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T89416 WP_000813794.1 NZ_CP024978:c4857291-4857115 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T89416 NZ_CP024978:c4857291-4857115 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT89416 WP_001270286.1 NZ_CP024978:c4857069-4856653 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT89416 NZ_CP024978:c4857069-4856653 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References