Detailed information of TA system TA089416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4856653..4857291
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CV83915_RS28690
• Protein ID: WP_000813794.1
• Coordinates: 4857115..4857291 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CV83915_RS28685
• Protein ID: WP_001270286.1
• Coordinates: 4856653..4857069 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS28665	4851805..4852746	-	942	WP_001251313.1	ABC transporter permease	-
CV83915_RS28670	4852747..4853760	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
CV83915_RS28675	4853778..4854923	-	1146	WP_063079343.1	ABC transporter substrate-binding protein	-
CV83915_RS28680	4855168..4856574	-	1407	WP_063079344.1	PLP-dependent aminotransferase family protein	-
CV83915_RS28685	4856653..4857069	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CV83915_RS28690	4857115..4857291	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CV83915_RS28695	4857513..4857743	+	231	WP_074150579.1	DUF2554 family protein	-
CV83915_RS28700	4857835..4859796	-	1962	WP_001619413.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CV83915_RS28705	4859869..4860405	-	537	WP_016244590.1	DNA-binding transcriptional regulator SutR	-
CV83915_RS28710	4860458..4861672	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4861712..4862860	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T89416 WP_000813794.1 NZ_CP024978:c4857291-4857115 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T89416 NZ_CP024978:c4857291-4857115 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT89416 WP_001270286.1 NZ_CP024978:c4857069-4856653 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT89416 NZ_CP024978:c4857069-4856653 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB