Detailed information of TA system TA089406

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4214835..4215670
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: CV83915_RS25065
• Protein ID: WP_000854760.1
• Coordinates: 4214835..4215212 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CV83915_RS25070
• Protein ID: WP_001295723.1
• Coordinates: 4215302..4215670 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS25035	4210406..4210753	-	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
CV83915_RS25040	4210753..4211403	-	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
CV83915_RS29545	4211986..4212357	-	372	WP_001295631.1	IS110 family transposase	-
CV83915_RS29550	4213173..4213286	-	114	WP_032140621.1	DUF957 domain-containing protein	-
CV83915_RS25060	4214656..4214838	-	183	WP_063079228.1	hypothetical protein	-
CV83915_RS25065	4214835..4215212	-	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
CV83915_RS25070	4215302..4215670	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CV83915_RS25075	4215833..4216054	-	222	WP_063079229.1	DUF987 domain-containing protein	-
CV83915_RS25080	4216117..4216593	-	477	WP_001186774.1	RadC family protein	-
CV83915_RS25085	4216609..4217082	-	474	WP_063079232.1	antirestriction protein	-
CV83915_RS25095	4217424..4218242	-	819	WP_063079230.1	DUF945 domain-containing protein	-
CV83915_RS25105	4218397..4218555	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4213339..4265077	51738

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T89406 WP_000854760.1 NZ_CP024978:c4215212-4214835 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T89406 NZ_CP024978:c4215212-4214835 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT89406 WP_001295723.1 NZ_CP024978:c4215670-4215302 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89406 NZ_CP024978:c4215670-4215302 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3

Antitoxin

• AlphaFold DB: S1NM52