Detailed information of TA system TA089404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3616704..3617329
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CV83915_RS21890
• Protein ID: WP_000911330.1
• Coordinates: 3616931..3617329 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CV83915_RS21885
• Protein ID: WP_000450524.1
• Coordinates: 3616704..3616931 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS21860	3612507..3612977	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CV83915_RS21865	3612977..3613549	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CV83915_RS21870	3613695..3614573	+	879	WP_016244934.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CV83915_RS21875	3614590..3615624	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
CV83915_RS21880	3615837..3616550	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CV83915_RS21885	3616704..3616931	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CV83915_RS21890	3616931..3617329	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CV83915_RS21895	3617476..3618339	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CV83915_RS21900	3618354..3620369	+	2016	WP_000829393.1	tRNA cytosine(34) acetyltransferase TmcA	-
CV83915_RS21905	3620443..3621141	+	699	WP_000679812.1	esterase	-
CV83915_RS21910	3621251..3621451	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T89404 WP_000911330.1 NZ_CP024978:3616931-3617329 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T89404 NZ_CP024978:3616931-3617329 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT89404 WP_000450524.1 NZ_CP024978:3616704-3616931 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT89404 NZ_CP024978:3616704-3616931 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33