Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3180517..3181177 Replicon chromosome
Accession NZ_CP024978
Organism Escherichia coli strain CV839-15

Toxin (Protein)


Gene name cptA Uniprot ID A0A398Q8H4
Locus tag CV83915_RS19590 Protein ID WP_000244782.1
Coordinates 3180764..3181177 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CV83915_RS19585 Protein ID WP_000354046.1
Coordinates 3180517..3180783 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83915_RS19560 3175686..3176429 + 744 WP_000951948.1 SDR family oxidoreductase -
CV83915_RS19565 3176486..3177919 - 1434 WP_032359762.1 6-phospho-beta-glucosidase BglA -
CV83915_RS19570 3177964..3178275 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CV83915_RS19575 3178439..3179098 + 660 WP_000250274.1 hemolysin III family protein -
CV83915_RS19580 3179294..3180274 - 981 WP_049589719.1 tRNA-modifying protein YgfZ -
CV83915_RS19585 3180517..3180783 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CV83915_RS19590 3180764..3181177 + 414 WP_000244782.1 protein YgfX Toxin
CV83915_RS19595 3181211..3181732 - 522 WP_001055872.1 flavodoxin FldB -
CV83915_RS19600 3181844..3182740 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
CV83915_RS19605 3182764..3183474 + 711 WP_016245061.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CV83915_RS19610 3183480..3185213 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16305.25 Da        Isoelectric Point: 11.7064

>T89401 WP_000244782.1 NZ_CP024978:3180764-3181177 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T89401 NZ_CP024978:3180764-3181177 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT89401 WP_000354046.1 NZ_CP024978:3180517-3180783 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT89401 NZ_CP024978:3180517-3180783 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A398Q8H4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References