Detailed information of TA system TA089400

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3028954..3029789
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2H4TUZ7
• Locus tag: CV83915_RS18695
• Protein ID: WP_000854744.1
• Coordinates: 3029412..3029789 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3J1F1Y9
• Locus tag: CV83915_RS18690
• Protein ID: WP_063104706.1
• Coordinates: 3028954..3029322 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS18650	3024192..3024674	+	483	WP_000649890.1	hypothetical protein	-
CV83915_RS18665	3025033..3025851	+	819	WP_001234708.1	DUF945 domain-containing protein	-
CV83915_RS29160	3026184..3026885	-	702	WP_137432245.1	hypothetical protein	-
CV83915_RS18675	3027278..3027742	+	465	WP_000131518.1	antirestriction protein	-
CV83915_RS18680	3027757..3028233	+	477	WP_001186727.1	RadC family protein	-
CV83915_RS18685	3028302..3028904	+	603	WP_000692332.1	hypothetical protein	-
CV83915_RS18690	3028954..3029322	+	369	WP_063104706.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CV83915_RS18695	3029412..3029789	+	378	WP_000854744.1	TA system toxin CbtA family protein	Toxin
CV83915_RS18700	3029786..3030274	+	489	WP_000777668.1	hypothetical protein	-
CV83915_RS18705	3030286..3030483	+	198	WP_000772034.1	DUF957 domain-containing protein	-
CV83915_RS18710	3030568..3031413	+	846	WP_000065753.1	DUF4942 domain-containing protein	-
CV83915_RS18720	3031713..3032219	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
CV83915_RS18725	3032298..3034139	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14138.13 Da
• Isoelectric Point: 8.5163

>T89400 WP_000854744.1 NZ_CP024978:3029412-3029789 [Escherichia coli]
MKTLPDTHVREASRCSSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACSCSQLINSIDILRARRATGLMTRSHYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T89400 NZ_CP024978:3029412-3029789 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCTCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTAAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTAGCTGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCCCGCCGGGCAACAGGTCTGATGACCCGCAG
CCACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13571.25 Da
• Isoelectric Point: 6.2044

>AT89400 WP_063104706.1 NZ_CP024978:3028954-3029322 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPSTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPCHQHTVTLYARGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89400 NZ_CP024978:3028954-3029322 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCAGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCTGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCAGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2H4TUZ7

Antitoxin

• AlphaFold DB: A0A3J1F1Y9