Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2588101..2588732 Replicon chromosome
Accession NZ_CP024978
Organism Escherichia coli strain CV839-15

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CV83915_RS16265 Protein ID WP_001259385.1
Coordinates 2588101..2588376 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CV83915_RS16270 Protein ID WP_000593555.1
Coordinates 2588373..2588732 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83915_RS16250 2583105..2584172 + 1068 WP_016243966.1 HlyD family secretion protein -
CV83915_RS16255 2584169..2586904 + 2736 WP_016243965.1 ribosome-associated ATPase/putative transporter RbbA -
CV83915_RS16260 2586904..2588028 + 1125 WP_001216257.1 ABC transporter permease -
CV83915_RS16265 2588101..2588376 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CV83915_RS16270 2588373..2588732 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CV83915_RS16275 2588793..2589490 - 698 Protein_2494 IS1 family transposase -
CV83915_RS16285 2589629..2590030 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CV83915_RS16290 2590036..2590842 - 807 WP_016243964.1 nickel import ATP-binding protein NikE -
CV83915_RS16295 2590839..2591603 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
CV83915_RS16300 2591603..2592436 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CV83915_RS16305 2592433..2593377 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2578506..2589170 10664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T89398 WP_001259385.1 NZ_CP024978:2588101-2588376 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T89398 NZ_CP024978:2588101-2588376 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT89398 WP_000593555.1 NZ_CP024978:2588373-2588732 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT89398 NZ_CP024978:2588373-2588732 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTATGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References