Detailed information of TA system TA089394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1082506..1083343
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: CV83915_RS08375
• Protein ID: WP_000227784.1
• Coordinates: 1082801..1083343 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CV83915_RS08370
• Protein ID: WP_001297137.1
• Coordinates: 1082506..1082817 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS08345	1077526..1078473	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
CV83915_RS08350	1078495..1080486	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CV83915_RS08355	1080476..1081090	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CV83915_RS08360	1081090..1081419	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CV83915_RS08365	1081431..1082321	+	891	WP_000971327.1	heme o synthase	-
CV83915_RS08370	1082506..1082817	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CV83915_RS08375	1082801..1083343	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
CV83915_RS08380	1083399..1083590	-	192	WP_063079746.1	hypothetical protein	-
CV83915_RS08385	1083663..1084313	+	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
CV83915_RS08390	1084313..1084660	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
CV83915_RS08395	1084680..1086251	+	1572	WP_000381442.1	IS66 family transposase	-
CV83915_RS08400	1086282..1087046	-	765	Protein_1099	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T89394 WP_000227784.1 NZ_CP024978:1082801-1083343 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T89394 NZ_CP024978:1082801-1083343 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT89394 WP_001297137.1 NZ_CP024978:1082506-1082817 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT89394 NZ_CP024978:1082506-1082817 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3