89389

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 174349..174874 Replicon plasmid pCV839-15-p2
Accession NZ_CP024976
Organism Escherichia coli strain CV839-15

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CV83915_RS01735 Protein ID WP_001159871.1
Coordinates 174569..174874 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CV83915_RS01730 Protein ID WP_000813630.1
Coordinates 174349..174567 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83915_RS01705 172087..173220 + 1134 WP_000545983.1 DUF3800 domain-containing protein -
CV83915_RS01710 173240..173524 + 285 WP_000642771.1 hypothetical protein -
CV83915_RS01715 173521..173718 - 198 WP_000215657.1 hypothetical protein -
CV83915_RS01730 174349..174567 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CV83915_RS01735 174569..174874 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CV83915_RS01740 174875..175681 + 807 WP_000016968.1 site-specific integrase -
CV83915_RS01745 175821..177034 + 1214 WP_162829786.1 IS3 family transposase -
CV83915_RS01755 177977..179182 + 1206 WP_001442122.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 - 1..195578 195578


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T89389 WP_001159871.1 NZ_CP024976:174569-174874 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T89389 NZ_CP024976:174569-174874 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT89389 WP_000813630.1 NZ_CP024976:174349-174567 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT89389 NZ_CP024976:174349-174567 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References