Detailed information of TA system TA089387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 104748..105295
• Replicon: plasmid pCV839-15-p2
• Accession: NZ_CP024976
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: CV83915_RS01320
• Protein ID: WP_000969996.1
• Coordinates: 105014..105295 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2H4TKU7
• Locus tag: CV83915_RS01315
• Protein ID: WP_000079923.1
• Coordinates: 104748..105017 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS01260	99850..100410	+	561	WP_063079260.1	fertility inhibition protein FinO	-
CV83915_RS01265	100545..100757	+	213	WP_063079259.1	hypothetical protein	-
CV83915_RS29310	101001..101087	+	87	Protein_117	endonuclease	-
CV83915_RS01280	101268..101579	-	312	WP_063079258.1	hypothetical protein	-
-	101875..101936	-	62	NuclAT_5	-	-
-	101875..101936	-	62	NuclAT_5	-	-
-	101875..101936	-	62	NuclAT_5	-	-
-	101875..101936	-	62	NuclAT_5	-	-
CV83915_RS01285	101923..102129	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	-
CV83915_RS01290	102413..102670	+	258	WP_000083845.1	replication regulatory protein RepA	-
CV83915_RS01300	102904..102978	+	75	WP_001375168.1	RepA leader peptide Tap	-
CV83915_RS01305	102971..103829	+	859	Protein_122	incFII family plasmid replication initiator RepA	-
CV83915_RS01315	104748..105017	+	270	WP_000079923.1	hypothetical protein	Antitoxin
CV83915_RS01320	105014..105295	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CV83915_RS01325	105335..105898	+	564	WP_063079397.1	hypothetical protein	-
CV83915_RS01335	106185..107189	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
CV83915_RS01340	107587..108393	-	807	Protein_127	DUF2219 family protein	-
CV83915_RS28990	108890..109081	+	192	WP_157895655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2	-	1..195578	195578
	-	flank	IS/Tn	-	-	106185..107189	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T89387 WP_000969996.1 NZ_CP024976:105014-105295 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T89387 NZ_CP024976:105014-105295 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10196.52 Da
• Isoelectric Point: 7.2050

>AT89387 WP_000079923.1 NZ_CP024976:104748-105017 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT89387 NZ_CP024976:104748-105017 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACAGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2H4TKU7