Detailed information of TA system TA089377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 66653..67200
• Replicon: plasmid pCV839-15-p1
• Accession: NZ_CP024975
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CV83915_RS00460
• Protein ID: WP_000970003.1
• Coordinates: 66919..67200 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2H4TKA5
• Locus tag: CV83915_RS00455
• Protein ID: WP_000079937.1
• Coordinates: 66653..66922 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS00415	61771..62370	+	600	WP_032083981.1	hypothetical protein	-
CV83915_RS00420	62534..62764	+	231	WP_001736714.1	hypothetical protein	-
CV83915_RS00425	63029..63283	+	255	WP_000083830.1	replication regulatory protein RepA	-
CV83915_RS00435	63519..63593	+	75	WP_001365705.1	RepA leader peptide Tap	-
CV83915_RS00440	63586..64443	+	858	WP_000130986.1	incFII family plasmid replication initiator RepA	-
CV83915_RS00450	65440..66477	+	1038	WP_032083970.1	DNA-binding protein	-
CV83915_RS00455	66653..66922	+	270	WP_000079937.1	hypothetical protein	Antitoxin
CV83915_RS00460	66919..67200	+	282	WP_000970003.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CV83915_RS00465	67509..68651	+	1143	WP_158649904.1	hypothetical protein	-
CV83915_RS00470	68740..69045	+	306	WP_000482654.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa	1..86835	86835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10863.48 Da
• Isoelectric Point: 8.4615

>T89377 WP_000970003.1 NZ_CP024975:66919-67200 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLTAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T89377 NZ_CP024975:66919-67200 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAC
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10174.56 Da
• Isoelectric Point: 10.1760

>AT89377 WP_000079937.1 NZ_CP024975:66653-66922 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT89377 NZ_CP024975:66653-66922 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCACGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2H4TKA5