Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1932575..1933187 Replicon chromosome
Accession NZ_CP024970
Organism Erwinia amylovora strain E-2

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag AD997_RS09095 Protein ID WP_012906750.1
Coordinates 1932575..1932754 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID D4I356
Locus tag AD997_RS09100 Protein ID WP_004157632.1
Coordinates 1932777..1933187 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD997_RS09075 1928133..1929083 + 951 WP_004157624.1 DUF1471 domain-containing protein -
AD997_RS09080 1929291..1930682 + 1392 WP_004157625.1 amino acid permease -
AD997_RS09085 1930686..1931150 - 465 WP_004157626.1 hypothetical protein -
AD997_RS09090 1931327..1932055 + 729 WP_004157629.1 two-component system response regulator RstA -
AD997_RS09095 1932575..1932754 + 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
AD997_RS09100 1932777..1933187 + 411 WP_004157632.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AD997_RS09105 1933243..1934244 + 1002 WP_004157633.1 acyltransferase -
AD997_RS09115 1935115..1935327 - 213 WP_004157635.1 hypothetical protein -
AD997_RS18335 1935585..1935758 - 174 WP_004157636.1 hypothetical protein -
AD997_RS18120 1935853..1936026 - 174 WP_013036045.1 hypothetical protein -
AD997_RS09120 1936037..1936309 - 273 WP_004157637.1 hypothetical protein -
AD997_RS09130 1936915..1937529 + 615 WP_004166850.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1932575..1939843 7268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T89361 WP_012906750.1 NZ_CP024970:1932575-1932754 [Erwinia amylovora]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T89361 NZ_CP024970:1932575-1932754 [Erwinia amylovora]
ATGGATAGCAGGAATGCAATAGCAATGATAGAGGCCGATGGATGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAGCACCCAACGAAAAAAGGGCTGGTAACGGTAAAACATCCCCAAAAGGACATACCGCTACCAACGCTGAAAAGCATTA
AAAAACAGGCGGGGCTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14829.76 Da        Isoelectric Point: 4.3623

>AT89361 WP_004157632.1 NZ_CP024970:1932777-1933187 [Erwinia amylovora]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESAREAIDAHIELLVEDGEAVPEATSVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Download         Length: 411 bp

>AT89361 NZ_CP024970:1932777-1933187 [Erwinia amylovora]
ATGTTATACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCCGATATTCCAGGCTGCTTCTCTGG
CGGCGACAATTATCAGGATGCGATTGAGAGCGCGCGTGAGGCTATCGACGCGCATATCGAATTACTGGTGGAAGATGGCG
AAGCGGTGCCGGAAGCGACCAGCGTGGAAAACTGGCTGGCCGATCCCGATTATGTTGGTGTGGTGTGGGCGCTGGTAGAT
GTGGACGTTACCCGGCTGATGGGGAAAGCGGAAAAAATCAATGTTACGCTCCCTTCTTTACTGATCCGCCGTATCGATCA
GTTTGTCGCTGCACATCCTGAGTACGGTAGCCGTTCCGGCTTCCTGTCCCGCGTGGCGGCTGATAAAGTGATTGGTCGCG
AAAAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A830ZVY0

References