Detailed information of TA system TA089359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1117765..1118392
• Replicon: chromosome
• Accession: NZ_CP024970
• Organism: Erwinia amylovora strain E-2

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: AD997_RS05175
• Protein ID: WP_004156337.1
• Coordinates: 1117765..1117983 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AD997_RS05180
• Protein ID: WP_013035913.1
• Coordinates: 1118003..1118392 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD997_RS05145	1113751..1114089	+	339	WP_004156319.1	P-II family nitrogen regulator	-
AD997_RS05150	1114107..1115408	+	1302	WP_004172214.1	ammonium transporter AmtB	-
AD997_RS05155	1115483..1116343	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
AD997_RS05160	1116568..1117125	+	558	WP_004156333.1	YbaY family lipoprotein	-
AD997_RS05165	1117148..1117465	-	318	WP_004156334.1	MGMT family protein	-
AD997_RS05175	1117765..1117983	-	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
AD997_RS05180	1118003..1118392	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
AD997_RS05185	1118539..1118892	-	354	WP_004156342.1	hypothetical protein	-
AD997_RS05200	1119664..1119810	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
AD997_RS05205	1119798..1120079	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
AD997_RS05210	1120227..1123373	-	3147	WP_004156353.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T89359 WP_004156337.1 NZ_CP024970:c1117983-1117765 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T89359 NZ_CP024970:c1117983-1117765 [Erwinia amylovora]
ATGACTGACAAACTTCTAACGAAAACTGACTACCTGATGCGCCTCAGGCGTTGTCGTTCAATCGATACACTTGAACGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCTGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTCGCTGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT89359 WP_013035913.1 NZ_CP024970:c1118392-1118003 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT89359 NZ_CP024970:c1118392-1118003 [Erwinia amylovora]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCATCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTTCAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACAATGAATTACAAAATCAAGCATATCGAAGACAGCGATCTGATAAGTCAAATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTACGGTGTCAACAATCTTGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCGTAATGAGCCAGATCCAGTCCAGCCATTCATTTAGCAGCCCATAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0