Detailed information of TA system TA089358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 903588..904041
• Replicon: chromosome
• Accession: NZ_CP024970
• Organism: Erwinia amylovora strain E-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AD997_RS04110
• Protein ID: WP_013035888.1
• Coordinates: 903805..904041 (+)
• Length: 79 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4HZA2
• Locus tag: AD997_RS04105
• Protein ID: WP_004159527.1
• Coordinates: 903588..903815 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AD997_RS04095	902413..902916	+	504	WP_048901601.1	hypothetical protein	-
AD997_RS04100	902980..903267	-	288	WP_004159528.1	SymE family type I addiction module toxin	-
AD997_RS04105	903588..903815	+	228	WP_004159527.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AD997_RS04110	903805..904041	+	237	WP_013035888.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AD997_RS04115	904028..904246	-	219	WP_004159522.1	hypothetical protein	-
AD997_RS04120	904574..906790	+	2217	Protein_748	ferrichrome porin FhuA	-
AD997_RS04125	906839..907633	+	795	WP_004159512.1	Fe3+-hydroxamate ABC transporter ATP-binding protein FhuC	-
AD997_RS04130	907648..908526	+	879	WP_004159509.1	Fe(3+)-hydroxamate ABC transporter substrate-binding protein FhuD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-78)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 79 a.a.
• Molecular weight: 9135.79 Da
• Isoelectric Point: 9.0876

>T89358 WP_013035888.1 NZ_CP024970:903805-904041 [Erwinia amylovora]
ISYELLFDPWALKEWKKLGATVREQFKKKLAEVLVNLQVESARLRDHPDCCKIKLKSSGYRLVYQVQDEQLVMLVISV

Nucleotide

• Length: 237 bp

>T89358 NZ_CP024970:903805-904041 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8435.90 Da
• Isoelectric Point: 7.9766

>AT89358 WP_004159527.1 NZ_CP024970:903588-903815 [Erwinia amylovora]
VRLDDKLKSKACAVLDELDVSPTEAVRLLFQYIAENERLPLKALTVSDEKDALLKTVRERLASLQKGIKVTLDKL

Nucleotide

• Length: 228 bp

>AT89358 NZ_CP024970:903588-903815 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A831A410