Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4077144..4077784 Replicon chromosome
Accession NZ_CP024956
Organism Pectobacterium atrosepticum strain 36A

Toxin (Protein)


Gene name vagD Uniprot ID A0A0J5XQX5
Locus tag CVS35_RS18500 Protein ID WP_039310134.1
Coordinates 4077144..4077560 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6D0W2
Locus tag CVS35_RS18505 Protein ID WP_011095187.1
Coordinates 4077557..4077784 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVS35_RS18480 4072965..4074296 + 1332 WP_039310142.1 type I-F CRISPR-associated protein Csy1 -
CVS35_RS18485 4074293..4075225 + 933 WP_039310139.1 type I-F CRISPR-associated protein Csy2 -
CVS35_RS18490 4075239..4076252 + 1014 WP_039310137.1 type I-F CRISPR-associated protein Csy3 -
CVS35_RS18495 4076262..4076816 + 555 WP_039310136.1 type I-F CRISPR-associated endoribonuclease Cas6/Csy4 -
CVS35_RS18500 4077144..4077560 - 417 WP_039310134.1 type II toxin-antitoxin system VapC family toxin Toxin
CVS35_RS18505 4077557..4077784 - 228 WP_011095187.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CVS35_RS18510 4078621..4079040 - 420 WP_011095188.1 secondary thiamine-phosphate synthase enzyme YjbQ -
CVS35_RS18515 4079058..4081892 - 2835 WP_039296673.1 excinuclease ABC subunit UvrA -
CVS35_RS18520 4082138..4082701 + 564 WP_011095190.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15201.90 Da        Isoelectric Point: 9.3444

>T89349 WP_039310134.1 NZ_CP024956:c4077560-4077144 [Pectobacterium atrosepticum]
VSRLFMLDTNICSFIMREHPAAVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMLLVDAFCRRLDAILPWDSA
AVDATTKIKIALAAAGTPIGPNDTAIAGHAISVKAVLVTNNLREFERVPGLMLEDWVK

Download         Length: 417 bp

>T89349 NZ_CP024956:c4077560-4077144 [Pectobacterium atrosepticum]
GTGAGCCGTTTGTTCATGCTTGATACTAATATCTGTTCTTTCATAATGCGTGAACACCCAGCTGCGGTGATTGAAAAGTT
GCAGCAATGCACATTGCGACGCGATCGAATCGTGGTATCAGCGATCACGTACGCTGAAATGCGTTTTGGGGCGGTTGGCA
AAAAAGCTTCGCCGCGCCATATGCTGTTGGTTGATGCTTTCTGCCGCCGACTTGATGCGATATTACCTTGGGATAGCGCA
GCGGTAGATGCCACCACAAAAATCAAAATCGCGTTAGCTGCCGCTGGTACGCCTATTGGCCCAAATGATACGGCCATCGC
TGGTCATGCTATCAGCGTTAAGGCCGTATTGGTCACCAATAATCTGAGAGAGTTTGAGCGAGTGCCAGGGCTGATGCTTG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8894.05 Da        Isoelectric Point: 4.5216

>AT89349 WP_011095187.1 NZ_CP024956:c4077784-4077557 [Pectobacterium atrosepticum]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEERADADFMTERQDVVEEGRFEL

Download         Length: 228 bp

>AT89349 NZ_CP024956:c4077784-4077557 [Pectobacterium atrosepticum]
ATGCGTATTGTCTCAGTATTTAAAAATGGTAATAATCGTGCTATTCGCTTACCGCGTGATATGGACTTTGAAGGCGTCAA
TGAACTGGAGATCGTGCGTGAAGGGGATACCATCATGTTGCGCCCAGTCAAACCCAATTGGCATTCGTTTCTGCAAGAAG
AGCGCGCTGACGCTGACTTTATGACAGAACGTCAGGATGTAGTCGAAGAGGGGAGATTCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J5XQX5

Antitoxin

Source ID Structure
AlphaFold DB A0A0J5XN10

References