Detailed information of TA system TA089345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 919398..919923
• Replicon: chromosome
• Accession: NZ_CP024956
• Organism: Pectobacterium atrosepticum strain 36A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CVS35_RS04255
• Protein ID: WP_039290137.1
• Coordinates: 919398..919682 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6D956
• Locus tag: CVS35_RS04260
• Protein ID: WP_005973616.1
• Coordinates: 919672..919923 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVS35_RS04230	914732..915193	-	462	Protein_795	hypothetical protein	-
CVS35_RS04235	916048..916680	-	633	WP_158296417.1	transglycosylase domain-containing protein	-
CVS35_RS22540	916617..917237	-	621	WP_158296418.1	hypothetical protein	-
CVS35_RS04240	917540..917919	-	380	Protein_798	winged helix-turn-helix domain-containing protein	-
CVS35_RS04245	918341..918886	+	546	WP_039282029.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
CVS35_RS04250	918870..919070	+	201	Protein_800	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CVS35_RS04255	919398..919682	-	285	WP_039290137.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CVS35_RS04260	919672..919923	-	252	WP_005973616.1	prevent-host-death protein	Antitoxin
CVS35_RS04265	920183..922510	-	2328	WP_039290139.1	toprim domain-containing protein	-
CVS35_RS04270	922521..922862	-	342	WP_039290142.1	DUF5375 family protein	-
CVS35_RS04275	922859..923125	-	267	WP_039290145.1	hypothetical protein	-
CVS35_RS04280	923122..923316	-	195	WP_039294697.1	hypothetical protein	-
CVS35_RS22545	923309..923506	-	198	WP_039294701.1	host cell division inhibitor Icd-like protein	-
CVS35_RS04285	923485..923853	-	369	Protein_808	ash family protein	-
CVS35_RS04290	923862..924086	-	225	WP_039281995.1	hypothetical protein	-
CVS35_RS04295	924083..924343	-	261	WP_039290147.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	857268..965108	107840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10907.88 Da
• Isoelectric Point: 10.1988

>T89345 WP_039290137.1 NZ_CP024956:c919682-919398 [Pectobacterium atrosepticum]
MSYKLEFEEHALKEFKKLGAPVRELFTKKLKAVLQNPHVPANRLHGMADCYKIKLRSAGYRLVYQVLEHEIVVLVLAVGK
RERSEVYQAAKDRL

Nucleotide

• Length: 285 bp

>T89345 NZ_CP024956:c919682-919398 [Pectobacterium atrosepticum]
ATGAGTTATAAGCTGGAGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGCTGGGTGCACCCGTGCGGGAGCTGTTCAC
CAAAAAGCTGAAAGCGGTTTTACAAAATCCCCACGTTCCCGCCAACCGACTTCATGGAATGGCTGACTGCTACAAAATCA
AACTTCGTTCCGCTGGCTATCGGCTAGTGTATCAAGTGCTTGAGCATGAGATCGTAGTACTGGTTTTAGCTGTGGGCAAG
CGTGAACGCTCAGAGGTTTATCAAGCAGCCAAAGACCGACTATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9079.35 Da
• Isoelectric Point: 5.0807

>AT89345 WP_005973616.1 NZ_CP024956:c919923-919672 [Pectobacterium atrosepticum]
MSVQPILANAAVSISDFKKSPNAALKEANGEPVAVLTNGRISGYYVSPETWEALADYQEDIELAKIARSRMKGKRVKVDL
DEL

Nucleotide

• Length: 252 bp

>AT89345 NZ_CP024956:c919923-919672 [Pectobacterium atrosepticum]
ATGTCCGTGCAACCTATTCTTGCCAATGCTGCCGTGAGTATTAGCGACTTTAAAAAATCGCCCAATGCAGCGTTGAAAGA
AGCTAACGGCGAGCCTGTAGCCGTGTTAACCAATGGCCGTATCTCTGGCTATTATGTTTCCCCGGAAACATGGGAAGCGC
TGGCCGACTATCAGGAAGATATCGAACTTGCCAAAATTGCCCGCTCCCGGATGAAGGGGAAACGTGTGAAGGTCGATCTG
GATGAGTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0G4JQP6