Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 194385..195038 Replicon chromosome
Accession NZ_CP024955
Organism Kyrpidia spormannii strain EA-1

Toxin (Protein)


Gene name mazF Uniprot ID D5WSA7
Locus tag CVV65_RS01105 Protein ID WP_013074285.1
Coordinates 194688..195038 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2K8N2G8
Locus tag CVV65_RS01100 Protein ID WP_041303528.1
Coordinates 194385..194684 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVV65_RS01080 189672..190064 + 393 WP_100669081.1 holo-ACP synthase -
CVV65_RS01085 190196..191707 + 1512 WP_100669082.1 NAD(P)H-hydrate dehydratase -
CVV65_RS01090 191788..192780 + 993 WP_133121180.1 outer membrane lipoprotein carrier protein LolA -
CVV65_RS01095 192791..193882 - 1092 WP_100666593.1 cation diffusion facilitator family transporter -
CVV65_RS01100 194385..194684 + 300 WP_041303528.1 CopG family ribbon-helix-helix protein Antitoxin
CVV65_RS01105 194688..195038 + 351 WP_013074285.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CVV65_RS01110 195098..196357 + 1260 WP_100666594.1 adenosylhomocysteinase -
CVV65_RS01115 196514..197254 + 741 WP_100669083.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
CVV65_RS01120 197546..197767 + 222 WP_013074288.1 DUF1659 domain-containing protein -
CVV65_RS01125 197803..198036 + 234 WP_100666595.1 DUF2922 domain-containing protein -
CVV65_RS01130 198130..198426 - 297 WP_198592074.1 zf-TFIIB domain-containing protein -
CVV65_RS01135 198545..199138 - 594 WP_100666597.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-53)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12906.94 Da        Isoelectric Point: 5.6976

>T89338 WP_013074285.1 NZ_CP024955:194688-195038 [Kyrpidia spormannii]
VNIKRGDIFFANLSPVVGSEQGGFRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGLDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVNESLMISLGLIDF

Download         Length: 351 bp

>T89338 NZ_CP024955:194688-195038 [Kyrpidia spormannii]
GTGAACATCAAGCGAGGGGATATTTTTTTTGCGAATCTATCCCCGGTGGTCGGTTCTGAACAAGGGGGCTTCCGGCCGGT
ATTGGTGATCCAAAACGATATCGGAAACCGGTTTAGCCCGACAGTCATCGTCGCGGCAATCACCGCCCAGATTCAGAAGG
CCAAGCTGCCCACCCACGTGGAGATTGATGCGAAGACCTACGGACTGGATCGGGATTCGGTCATCCTCCTGGAGCAGATT
CGCACCATTGATAAGCAGCGGTTGACGGATAAGATTACGCACCTGGACGATGAGATGATGAGCAAGGTCAACGAGTCGCT
CATGATCAGCCTGGGGTTGATCGATTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11329.99 Da        Isoelectric Point: 4.9577

>AT89338 WP_041303528.1 NZ_CP024955:194385-194684 [Kyrpidia spormannii]
MASVPEVLGLSETKRIMISVPSHLLQEVDGIAERENSNRSEVIRQAMRLYLEERKKERIRELMQRGYVEMAKINLYIASE
AFYAEEEAGSTLDRLVSGV

Download         Length: 300 bp

>AT89338 NZ_CP024955:194385-194684 [Kyrpidia spormannii]
ATGGCGAGTGTCCCGGAGGTGCTTGGGTTGTCCGAGACAAAGCGGATTATGATTAGTGTTCCGAGTCACCTGCTCCAGGA
AGTCGACGGAATTGCAGAAAGAGAGAATTCCAATCGAAGCGAGGTCATTCGCCAAGCGATGAGACTGTATTTGGAAGAGC
GTAAAAAGGAACGCATTCGCGAGTTGATGCAGCGGGGTTATGTGGAGATGGCGAAGATTAACCTTTATATTGCGTCGGAG
GCGTTTTACGCCGAAGAGGAGGCGGGTTCGACCCTAGATCGACTAGTAAGCGGGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K8N5K4

Antitoxin

Source ID Structure
AlphaFold DB A0A2K8N2G8

References