Detailed information of TA system TA089328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapD-relB/-
• Location: 260552..261209
• Replicon: chromosome
• Accession: NZ_CP024949
• Organism: Helicobacter pylori strain B136A

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: T5D7N9
• Locus tag: CV727_RS01270
• Protein ID: WP_000271050.1
• Coordinates: 260925..261209 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CV727_RS01265
• Protein ID: WP_145819958.1
• Coordinates: 260552..260944 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV727_RS01245	256153..256581	+	429	WP_108271155.1	30S ribosomal protein S6	-
CV727_RS01250	256591..257136	+	546	WP_000482429.1	single-stranded DNA-binding protein	-
CV727_RS01255	257159..257416	+	258	WP_000440196.1	30S ribosomal protein S18	-
CV727_RS01260	257988..260156	+	2169	WP_145819956.1	Hop family adhesin BabA	-
CV727_RS01265	260552..260944	+	393	WP_145819958.1	hypothetical protein	Antitoxin
CV727_RS01270	260925..261209	+	285	WP_000271050.1	endoribonuclease VapD	Toxin
CV727_RS01275	261377..261667	+	291	WP_145819960.1	hypothetical protein	-
CV727_RS01280	261681..261935	+	255	WP_145819962.1	type II toxin-antitoxin system YafQ family toxin	-
CV727_RS01285	262056..262580	-	525	WP_001135623.1	acyl-CoA thioesterase	-
CV727_RS01290	262739..263581	+	843	WP_145819964.1	SDR family oxidoreductase	-
CV727_RS01295	263574..264554	+	981	WP_000921461.1	iron ABC transporter permease	-
CV727_RS01300	264554..265321	+	768	WP_000242349.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11188.74 Da
• Isoelectric Point: 4.8896

>T89328 WP_000271050.1 NZ_CP024949:260925-261209 [Helicobacter pylori]
MYALAFDLKIEILKKEYGEPYNKAYDDLRQELELLGFEWTQGSVYVNYSKENTLAQVYKAINKLSQIEWFKKSVRDIRAF
KVEDFSDFTEIVKS

Nucleotide

• Length: 285 bp

>T89328 NZ_CP024949:260925-261209 [Helicobacter pylori]
ATGTATGCTCTAGCGTTTGATTTAAAGATTGAGATTTTAAAAAAAGAATACGGAGAACCCTACAATAAAGCCTATGATGA
TTTAAGGCAAGAATTAGAGCTATTAGGGTTTGAATGGACTCAAGGGAGCGTTTATGTTAATTATTCTAAAGAAAACACTC
TAGCACAAGTCTATAAAGCGATCAATAAACTCTCTCAAATTGAGTGGTTTAAAAAGTCTGTTAGGGATATTAGAGCGTTT
AAGGTAGAGGACTTTAGCGATTTTACTGAGATTGTGAAATCCTAG

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15240.39 Da
• Isoelectric Point: 9.5046

>AT89328 WP_145819958.1 NZ_CP024949:260552-260944 [Helicobacter pylori]
MPNTTAKKDYTKYSEKQLFNLIHRLERKIKKMQNDRVSFKEKMAKELEKRDQNFRDKIDALNELLQKISQAFDDKRDCCL
GRKIPNIQTQQAMKDAVNGVNLTQIDSLDDLTNELKRENNKGFENVCSSV

Nucleotide

• Length: 393 bp

>AT89328 NZ_CP024949:260552-260944 [Helicobacter pylori]
ATGCCTAACACCACCGCCAAAAAAGACTACACAAAATACAGCGAAAAACAGCTTTTTAACTTAATCCATCGATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAGTTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAGGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACATTCAAACGCAACAAGCCATGAAAGATGCGGTGAATGGGGTTAATCTCACTCAAATTGATAG
TTTAGATGATCTTACAAACGAGTTAAAAAGAGAAAATAATAAAGGTTTTGAAAATGTATGCTCTAGCGTTTGA

Structures

Toxin

• PDB: 3UI3