Detailed information of TA system TA089316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3134210..3134830
• Replicon: chromosome
• Accession: NZ_CP024944
• Organism: Burkholderia lata strain A05

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CVS37_RS33875
• Protein ID: WP_122949086.1
• Coordinates: 3134513..3134830 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: CVS37_RS33870
• Protein ID: WP_021157290.1
• Coordinates: 3134210..3134509 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVS37_RS33845	3129370..3130641	+	1272	WP_122949727.1	Rieske 2Fe-2S domain-containing protein	-
CVS37_RS33850	3130659..3131144	+	486	WP_059521536.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
CVS37_RS33855	3131165..3131491	+	327	WP_122949083.1	non-heme iron oxygenase ferredoxin subunit	-
CVS37_RS33860	3131481..3132701	+	1221	WP_122949084.1	FAD-dependent oxidoreductase	-
CVS37_RS33865	3132875..3134161	+	1287	WP_122949085.1	DUF445 domain-containing protein	-
CVS37_RS33870	3134210..3134509	-	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
CVS37_RS33875	3134513..3134830	-	318	WP_122949086.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CVS37_RS33880	3135180..3136820	+	1641	WP_122949087.1	acetolactate synthase large subunit	-
CVS37_RS33885	3136850..3138283	+	1434	WP_122949088.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-100)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11751.55 Da
• Isoelectric Point: 9.6019

>T89316 WP_122949086.1 NZ_CP024944:c3134830-3134513 [Burkholderia lata]
MPYCAPTFSIRTTGVFDAWFAGLPDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVVLL
CGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T89316 NZ_CP024944:c3134830-3134513 [Burkholderia lata]
ATGCCCTACTGTGCGCCTACGTTCAGCATCCGGACCACCGGCGTCTTCGACGCCTGGTTTGCCGGCCTGCCGGATCGCGT
CGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTCTCGATGGGCAATCCGGGCGACTGGAAGTCCGCCGGCTCGCCGG
TCGTCGAGATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGAACGATCTGGGTCGTCCTGCTC
TGCGGTGGCGATAAATCGACGCAACAGGCCGACATCCGCGCCGCCCACGCGATGCTCGCGCATCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT89316 WP_021157290.1 NZ_CP024944:c3134509-3134210 [Burkholderia lata]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT89316 NZ_CP024944:c3134509-3134210 [Burkholderia lata]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAAACCGAAGACGACATGGCCGACTACTTCGAAGC
ATGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCACGCGGCATGTCGC
AGGTCGCCCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCACTCGGCCTGCAACTGCACGGCGCAAAAGCGCACGCATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3