Detailed information of TA system TA089308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2958669..2959246
• Replicon: chromosome
• Accession: NZ_CP024942
• Organism: Paraburkholderia terricola strain mHS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CUJ90_RS31835
• Protein ID: WP_114164618.1
• Coordinates: 2958881..2959246 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1M6UE59
• Locus tag: CUJ90_RS31830
• Protein ID: WP_073431122.1
• Coordinates: 2958669..2958887 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ90_RS31815	2954807..2955394	+	588	WP_114164616.1	transcriptional regulator BetI	-
CUJ90_RS31820	2955423..2956892	+	1470	WP_114164617.1	betaine-aldehyde dehydrogenase	-
CUJ90_RS31825	2956905..2958590	+	1686	WP_073431121.1	choline dehydrogenase	-
CUJ90_RS31830	2958669..2958887	+	219	WP_073431122.1	ribbon-helix-helix protein, CopG family	Antitoxin
CUJ90_RS31835	2958881..2959246	+	366	WP_114164618.1	type II toxin-antitoxin system VapC family toxin	Toxin
CUJ90_RS31840	2959321..2960739	-	1419	WP_073431124.1	MFS transporter	-
CUJ90_RS31845	2960884..2961147	-	264	WP_073431125.1	hypothetical protein	-
CUJ90_RS31850	2961205..2961861	-	657	WP_114164619.1	sarcosine oxidase subunit gamma	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-103)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-41)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13444.64 Da
• Isoelectric Point: 9.4364

>T89308 WP_114164618.1 NZ_CP024942:2958881-2959246 [Paraburkholderia terricola]
MVKALFDTNILIDYLGGVGGAKKELARYEYRAISIITWMEVLVGATPEDEASIRGWLGSFDVIALDSTIANRAVEIRKQK
RIRLPDAIVWASAQVNSLLLVSRNVKDFPANEPGVRVPYKI

Nucleotide

• Length: 366 bp

>T89308 NZ_CP024942:2958881-2959246 [Paraburkholderia terricola]
ATGGTAAAAGCTCTTTTCGACACAAACATCCTGATCGACTATCTCGGCGGCGTCGGCGGAGCGAAGAAAGAGCTTGCGCG
CTATGAGTATCGAGCGATCAGCATCATCACATGGATGGAAGTTCTTGTTGGGGCAACCCCGGAAGACGAAGCCTCGATTC
GCGGATGGCTTGGCTCGTTCGACGTGATTGCGCTGGACAGCACAATTGCAAACCGTGCGGTTGAGATTCGCAAACAGAAG
CGCATCCGTCTGCCCGACGCGATTGTTTGGGCGTCGGCGCAGGTGAATTCCTTGCTGCTGGTATCGCGCAATGTCAAGGA
CTTTCCTGCGAACGAACCCGGCGTTCGAGTGCCTTACAAAATCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8434.63 Da
• Isoelectric Point: 5.1053

>AT89308 WP_073431122.1 NZ_CP024942:2958669-2958887 [Paraburkholderia terricola]
MSRILIDLPDSQVEELALLVESEQRPRAAVIRDAIEAYIAQHKRVLGNDVFGLWKNRKVDGLEYQRELRSEW

Nucleotide

• Length: 219 bp

>AT89308 NZ_CP024942:2958669-2958887 [Paraburkholderia terricola]
ATGAGCCGGATTCTGATTGATCTGCCCGATTCGCAGGTTGAAGAACTGGCATTGCTCGTAGAGTCGGAGCAACGCCCTCG
CGCCGCGGTTATTCGCGACGCAATCGAGGCGTATATCGCCCAGCATAAGCGCGTGCTGGGAAATGACGTTTTCGGACTGT
GGAAAAACAGAAAAGTCGATGGCCTTGAGTACCAGCGAGAATTGCGCTCGGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1M6UE59