Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 379922..380499 Replicon plasmid pPHS1_P
Accession NZ_CP024936
Organism Paraburkholderia graminis strain PHS1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CUJ91_RS33405 Protein ID WP_114158416.1
Coordinates 380134..380499 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag CUJ91_RS33400 Protein ID WP_107708214.1
Coordinates 379922..380140 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUJ91_RS33385 376165..377526 + 1362 WP_114158414.1 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme -
CUJ91_RS33390 377531..379054 + 1524 WP_114158415.1 amidase -
CUJ91_RS33400 379922..380140 + 219 WP_107708214.1 ribbon-helix-helix protein, CopG family Antitoxin
CUJ91_RS33405 380134..380499 + 366 WP_114158416.1 type II toxin-antitoxin system VapC family toxin Toxin
CUJ91_RS33410 380507..380737 + 231 WP_107708210.1 hypothetical protein -
CUJ91_RS33415 380749..381258 + 510 WP_107708208.1 restriction endonuclease -
CUJ91_RS34075 381289..381474 + 186 WP_132384582.1 hypothetical protein -
CUJ91_RS33420 381487..381774 + 288 WP_114158417.1 hypothetical protein -
CUJ91_RS33425 381782..382036 + 255 WP_114158418.1 hypothetical protein -
CUJ91_RS34565 382751..382990 + 240 WP_167450705.1 HNH endonuclease -
CUJ91_RS34245 383235..383816 - 582 WP_161966620.1 hypothetical protein -
CUJ91_RS33440 383840..384568 + 729 WP_114158420.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..494503 494503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(4-39)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13491.65 Da        Isoelectric Point: 8.4119

>T89293 WP_114158416.1 NZ_CP024936:380134-380499 [Paraburkholderia graminis]
MVKALFDTNILIDYLNGVGAAKKELARYEYRAISVITWMEVLVGASAEEDAAIRAWLDSFDVIAVDSAIANRAVEIRRTK
KIRLPDAIVWASAQVRSLLLVSRNTKDFPADEPGVRVPYKI

Download         Length: 366 bp

>T89293 NZ_CP024936:380134-380499 [Paraburkholderia graminis]
ATGGTGAAGGCGCTCTTCGATACGAACATCCTCATTGACTATCTCAACGGGGTCGGCGCAGCCAAGAAGGAACTGGCGCG
CTATGAGTATCGCGCGATCAGCGTGATCACCTGGATGGAAGTGCTGGTCGGAGCATCCGCCGAGGAAGACGCCGCGATCC
GCGCATGGCTCGATTCATTCGATGTCATCGCAGTAGACAGTGCGATCGCCAATCGCGCCGTCGAGATTCGCAGGACTAAA
AAGATTCGTCTGCCCGATGCCATCGTGTGGGCGTCAGCACAGGTCCGCTCGCTGTTGCTTGTTTCGCGAAATACGAAGGA
CTTTCCAGCAGACGAGCCGGGTGTCCGCGTGCCGTACAAAATTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8301.47 Da        Isoelectric Point: 5.0807

>AT89293 WP_107708214.1 NZ_CP024936:379922-380140 [Paraburkholderia graminis]
MSRILVDLPESQVDDLAVIVEAEQRPRAAVIRDAIEAYISQRKRALGTEVFGLWKSRKTDGLVYQQELRSEW

Download         Length: 219 bp

>AT89293 NZ_CP024936:379922-380140 [Paraburkholderia graminis]
ATGAGTCGTATCCTGGTTGACCTGCCTGAGTCGCAGGTTGACGACCTCGCTGTGATTGTGGAGGCCGAACAGCGGCCCCG
CGCAGCTGTCATCCGCGATGCCATTGAGGCGTATATCTCGCAGCGCAAGCGCGCGCTCGGAACAGAAGTATTCGGCCTGT
GGAAGAGCCGGAAGACGGATGGCCTTGTGTACCAGCAGGAGCTTCGTTCCGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References