Detailed information of TA system TA089288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3828881..3829470
• Replicon: chromosome
• Accession: NZ_CP024934
• Organism: Paraburkholderia graminis strain PHS1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CUJ91_RS17260
• Protein ID: WP_006050915.1
• Coordinates: 3828881..3829063 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B1G4G6
• Locus tag: CUJ91_RS17265
• Protein ID: WP_006050914.1
• Coordinates: 3829060..3829470 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ91_RS17235	3824569..3824994	-	426	WP_029970946.1	flagellar basal body rod protein FlgC	-
CUJ91_RS17240	3825108..3825599	-	492	WP_006050919.1	flagellar basal body rod protein FlgB	-
CUJ91_RS17245	3825816..3827414	+	1599	WP_006050918.1	flagellar basal body P-ring formation protein FlgA	-
CUJ91_RS17250	3827568..3827921	+	354	WP_006050917.1	flagellar biosynthesis anti-sigma factor FlgM	-
CUJ91_RS17255	3828016..3828465	+	450	WP_006050916.1	flagellar protein FlgN	-
CUJ91_RS17260	3828881..3829063	+	183	WP_006050915.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUJ91_RS17265	3829060..3829470	+	411	WP_006050914.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUJ91_RS17270	3829620..3830354	-	735	WP_006050913.1	RNA polymerase sigma factor FliA	-
CUJ91_RS17275	3830383..3831252	-	870	WP_029970951.1	flagellar biosynthesis protein FlhG	-
CUJ91_RS17280	3831245..3833266	-	2022	WP_114156558.1	flagellar biosynthesis protein FlhF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-129)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6610.79 Da
• Isoelectric Point: 12.1412

>T89288 WP_006050915.1 NZ_CP024934:3828881-3829063 [Paraburkholderia graminis]
MNSGEVVKLIHAAGWRLIRISGSHHHFRHAFKAGLVTIPHPKRDLPPGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T89288 NZ_CP024934:3828881-3829063 [Paraburkholderia graminis]
ATGAACAGCGGCGAGGTAGTCAAACTGATTCACGCGGCCGGTTGGCGGCTAATCCGAATATCGGGCAGCCATCACCATTT
CCGGCACGCATTCAAAGCCGGGCTCGTGACCATCCCTCATCCAAAAAGGGATCTTCCGCCCGGCACGCTGAACAGCATCT
TGAAACAGGCAGGGCTGAAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14797.74 Da
• Isoelectric Point: 4.7879

>AT89288 WP_006050914.1 NZ_CP024934:3829060-3829470 [Paraburkholderia graminis]
MKNLIFPVGIEAGDAQHAFGVVVPDIPGCHSAGDTLEEAYANAKEAIEAHLDTLLDEGLPIPERLTLDEHRRNPDYAGFT
WGFVTTRNIPALKKAVRINISLPEALVHEIDKYAQAHGMSRSAFLALAAEHEMADA

Nucleotide

• Length: 411 bp

>AT89288 NZ_CP024934:3829060-3829470 [Paraburkholderia graminis]
ATGAAAAACTTGATCTTTCCGGTCGGCATCGAGGCGGGCGACGCACAGCACGCGTTCGGCGTCGTCGTGCCGGATATTCC
GGGCTGCCATTCGGCGGGAGACACGCTGGAAGAGGCTTACGCGAACGCGAAAGAAGCGATCGAGGCGCATCTGGATACGC
TGCTCGACGAAGGCTTGCCGATTCCCGAAAGACTGACGCTGGACGAGCATCGGCGTAATCCTGACTATGCGGGTTTCACG
TGGGGCTTCGTGACGACCCGGAACATTCCCGCGCTCAAGAAGGCGGTGCGCATCAACATCTCGTTACCCGAGGCGTTGGT
GCATGAGATCGACAAATACGCGCAGGCGCACGGCATGTCGCGTTCCGCTTTTCTCGCGCTCGCGGCCGAGCACGAAATGG
CGGACGCTTGA

Structures

Antitoxin

• AlphaFold DB: B1G4G6