Detailed information of TA system TA089287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3257705..3258339
• Replicon: chromosome
• Accession: NZ_CP024934
• Organism: Paraburkholderia graminis strain PHS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CUJ91_RS14510
• Protein ID: WP_114156439.1
• Coordinates: 3257705..3258106 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: B1G264
• Locus tag: CUJ91_RS14515
• Protein ID: WP_006050036.1
• Coordinates: 3258106..3258339 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ91_RS14490	3253211..3254458	-	1248	WP_114156437.1	tRNA epoxyqueuosine(34) reductase QueG	-
CUJ91_RS14495	3254476..3255039	+	564	WP_114156438.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
CUJ91_RS14500	3255021..3256649	+	1629	WP_029968114.1	N-acetylmuramoyl-L-alanine amidase	-
CUJ91_RS14505	3256738..3257619	-	882	WP_006050038.1	O-acetylserine/cysteine exporter	-
CUJ91_RS14510	3257705..3258106	-	402	WP_114156439.1	type II toxin-antitoxin system VapC family toxin	Toxin
CUJ91_RS14515	3258106..3258339	-	234	WP_006050036.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CUJ91_RS14520	3258513..3259388	+	876	WP_107708656.1	pirin family protein	-
CUJ91_RS14525	3259497..3260345	+	849	WP_006050033.1	thioredoxin	-
CUJ91_RS14530	3260388..3261239	-	852	WP_114156440.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
CUJ91_RS14535	3261238..3261441	+	204	WP_149029195.1	hypothetical protein	-
CUJ91_RS14540	3261477..3262118	+	642	WP_006050031.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (6-55)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14864.06 Da
• Isoelectric Point: 4.9267

>T89287 WP_114156439.1 NZ_CP024934:c3258106-3257705 [Paraburkholderia graminis]
MPRYMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITFAELEYGVAVSANRARERRNLAALVEDILVAPFDSAAASA
YGPIQEATRERKKDQLDKLIAAHAVSLDVVLVTNNERDLTAYPGLRIENWLNA

Nucleotide

• Length: 402 bp

>T89287 NZ_CP024934:c3258106-3257705 [Paraburkholderia graminis]
ATGCCGCGCTACATGCTGGACACCAACATGTGCATCTACCTGATGAAGAATCAGCCGGAGCAGGTTGCGAAGCGTTTTGC
GGAATGCTTCGTCGGCGACGTCGTGATGTCCGCCATTACTTTCGCCGAGCTGGAGTACGGCGTGGCGGTCTCGGCCAATC
GTGCGCGCGAGCGTCGCAACCTTGCGGCGCTTGTCGAAGACATCCTGGTTGCGCCGTTCGACAGCGCGGCGGCGAGTGCC
TACGGCCCGATCCAGGAAGCCACGCGCGAGCGCAAGAAGGACCAGCTCGACAAGCTGATCGCGGCCCACGCGGTCTCGCT
CGACGTCGTGCTCGTCACCAACAATGAGCGCGATTTGACGGCCTATCCCGGCCTGCGTATCGAAAACTGGCTCAATGCCT
GA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8775.98 Da
• Isoelectric Point: 5.8652

>AT89287 WP_006050036.1 NZ_CP024934:c3258339-3258106 [Paraburkholderia graminis]
MLTKVFRNGNSQAVRIPAELAFSSVDMEVEIERVGDELRIRPTRRPLAGVLKKFSKFSPDFMAEGRGDHEQAEREDL

Nucleotide

• Length: 234 bp

>AT89287 NZ_CP024934:c3258339-3258106 [Paraburkholderia graminis]
ATGCTGACCAAGGTTTTTCGCAATGGCAATTCGCAGGCTGTGCGCATTCCGGCTGAACTGGCGTTTAGCAGCGTCGACAT
GGAAGTGGAAATCGAGCGGGTCGGCGACGAACTGCGCATTCGTCCCACGCGCCGCCCGCTCGCCGGCGTACTAAAAAAAT
TCTCGAAGTTCAGCCCGGATTTCATGGCGGAAGGCCGCGGCGACCACGAGCAGGCTGAGCGCGAGGATCTGTAA

Structures

Antitoxin

• AlphaFold DB: B1G264