Detailed information of TA system TA089285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4130650..4132373
• Replicon: chromosome
• Accession: NZ_CP024930
• Organism: Aeromonas veronii strain Aeromonas veronii X11

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: CVS41_RS18815
• Protein ID: WP_100654651.1
• Coordinates: 4130650..4131300 (+)
• Length: 217 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: CVS41_RS18820
• Protein ID: WP_100654652.1
• Coordinates: 4131300..4132373 (+)
• Length: 358 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVS41_RS19670	4125895..4127247	+	1353	WP_158651148.1	restriction endonuclease subunit S	-
CVS41_RS18805	4127244..4128623	+	1380	WP_100654649.1	DUF262 domain-containing protein	-
CVS41_RS18810	4128613..4130631	+	2019	WP_100654650.1	DUF262 domain-containing HNH endonuclease family protein	-
CVS41_RS18815	4130650..4131300	+	651	WP_100654651.1	DUF4433 domain-containing protein	Toxin
CVS41_RS18820	4131300..4132373	+	1074	WP_100654652.1	macro domain-containing protein	Antitoxin
CVS41_RS18825	4132598..4135708	+	3111	WP_100654653.1	HsdR family type I site-specific deoxyribonuclease	-
CVS41_RS18830	4135705..4136433	+	729	WP_100654654.1	M48 family metallopeptidase	-
CVS41_RS18835	4136550..4137353	-	804	WP_158651149.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4116674..4139760	23086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 217 a.a.
• Molecular weight: 25162.62 Da
• Isoelectric Point: 6.6581

>T89285 WP_100654651.1 NZ_CP024930:4130650-4131300 [Aeromonas veronii]
MPYDYSRSLNPEKALIFRIVHRDNLPWILTHGLWAGNAPEQSDTWVHIGLAELINKRAHHPVPLAPFGVLNDYVPFYFTP
FSPMLSNIHSGRGVRQRRNDEILILVSSLHRIAELELAFLFTDSHAYYQWANFYDDLGELNRIDWPILQRRDFKRDPDDP
AKFERYQAEALVHQHCPVEALVGVVCYNEETKAKVEQTMAQLGVARPVYVRSGWYF

Nucleotide

• Length: 651 bp

>T89285 NZ_CP024930:4130650-4131300 [Aeromonas veronii]
ATGCCATATGACTACAGTCGCAGTCTCAATCCAGAGAAGGCGCTGATCTTTCGTATTGTGCACCGTGACAATCTGCCATG
GATTTTGACCCATGGGCTGTGGGCGGGCAATGCGCCCGAGCAGAGTGATACCTGGGTACATATAGGCTTGGCCGAGTTGA
TCAACAAGCGCGCCCATCATCCGGTGCCACTGGCGCCATTTGGGGTGCTTAACGACTATGTGCCCTTCTACTTCACCCCT
TTCTCCCCAATGCTCAGCAATATCCACTCAGGGAGAGGTGTGCGGCAGCGCCGTAATGACGAGATCCTGATCCTGGTATC
CAGCCTGCATCGCATTGCTGAGCTGGAGCTGGCGTTTTTGTTTACCGACAGCCATGCCTATTATCAATGGGCCAATTTCT
ACGATGACCTGGGTGAACTGAACCGCATCGACTGGCCTATTTTGCAGCGTCGAGACTTCAAGCGTGACCCGGATGACCCT
GCCAAGTTTGAGCGGTATCAGGCAGAGGCGTTGGTTCATCAACACTGCCCCGTTGAGGCATTAGTCGGCGTGGTGTGTTA
CAACGAAGAGACAAAGGCTAAGGTTGAGCAAACGATGGCCCAATTGGGTGTCGCCCGACCGGTTTATGTTCGTTCTGGAT
GGTATTTCTGA

Antitoxin

Protein

• Length: 358 a.a.
• Molecular weight: 40362.52 Da
• Isoelectric Point: 7.8908

>AT89285 WP_100654652.1 NZ_CP024930:4131300-4132373 [Aeromonas veronii]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMARYAAACKSGQVTTGKMFITETGELLGPRWIINFPTK
QHWRSPSQMAWIQEGLQDLRHWLVQNHVKSIAIPPLGAGNGGLPWPEVKQAIEEALGDLDIEILLFEPSNQYQNVAKRTG
VEHLTPARALIAELVRRYWFLGMECSLLEIQKLAWFLERFIRQTAPQDPLQLHFTAHHYGPYANNLAHLLNAMDGSYLKS
DKRIPDSKPFDVIWFNDDKKQQLATYLHTEARAYLPALEQAAALIDGFESPYGMELLATVDWLVNQEGCKPTVEALRAGL
IRWPKGGKWAQRKAKLFDDKSLGIALTRLQQSFPAYH

Nucleotide

• Length: 1074 bp

>AT89285 NZ_CP024930:4131300-4132373 [Aeromonas veronii]
ATGATTCGATACACCACTGGCAACCTGCTAGATGCCCCTGTAGAGGCATTGGTGAATACAGTGAACACCGTTGGTGTCAT
GGGTAAAGGCATTGCGCTGATGTTTAAAGAGCGCTTCCCCGACAACATGGCTCGCTATGCGGCTGCCTGTAAGTCAGGGC
AAGTGACCACTGGCAAGATGTTCATCACCGAAACTGGTGAGCTACTCGGCCCCCGCTGGATCATCAACTTTCCCACCAAG
CAACATTGGCGATCTCCATCCCAGATGGCATGGATTCAGGAAGGCTTACAAGATCTGCGACATTGGCTTGTGCAAAATCA
CGTCAAATCTATCGCCATCCCCCCGCTAGGAGCAGGCAATGGTGGCCTACCATGGCCTGAAGTAAAACAGGCCATTGAAG
AGGCTCTCGGTGATCTGGATATTGAAATCCTGTTGTTTGAACCCAGCAACCAGTACCAAAATGTTGCCAAACGTACTGGC
GTCGAACATCTCACACCAGCCCGCGCCTTGATTGCCGAACTGGTCCGTCGCTACTGGTTTCTTGGCATGGAGTGCAGCCT
GCTGGAAATTCAAAAACTGGCATGGTTTCTGGAGCGCTTTATTCGTCAAACGGCGCCACAAGATCCGCTGCAGTTGCACT
TCACGGCACACCATTACGGGCCTTATGCCAATAATCTAGCCCACTTGCTGAATGCAATGGACGGCAGTTATCTCAAATCA
GATAAGCGGATCCCAGACAGCAAACCCTTTGATGTCATCTGGTTCAATGATGACAAAAAGCAACAACTTGCCACCTATTT
GCATACCGAGGCGCGAGCTTACTTACCCGCGCTGGAGCAAGCTGCTGCGCTTATCGACGGATTTGAGTCCCCCTATGGAA
TGGAGCTGCTAGCAACCGTTGACTGGCTGGTAAACCAAGAAGGATGCAAACCAACCGTAGAGGCGCTGCGGGCAGGACTT
ATCCGTTGGCCCAAAGGTGGCAAATGGGCACAACGTAAGGCCAAGCTGTTCGATGACAAGAGCTTGGGCATTGCCCTGAC
CCGCTTGCAGCAGAGCTTCCCTGCATATCACTGA