Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 18547..19139 Replicon plasmid pKPNH54.3
Accession NZ_CP024919
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag CU643_RS28640 Protein ID WP_000520549.1
Coordinates 18547..18795 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag CU643_RS28645 Protein ID WP_000681613.1
Coordinates 18792..19139 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS28935 15424..15939 + 516 WP_001025395.1 J domain-containing protein -
CU643_RS28610 16044..16586 + 543 WP_001083906.1 hypothetical protein -
CU643_RS28615 16583..16834 + 252 WP_001230707.1 hypothetical protein -
CU643_RS28620 16821..17048 + 228 WP_000801440.1 hypothetical protein -
CU643_RS28630 17502..17801 + 300 WP_000650309.1 hypothetical protein -
CU643_RS28635 17892..18242 + 351 WP_000854259.1 hypothetical protein -
CU643_RS28640 18547..18795 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
CU643_RS28645 18792..19139 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CU643_RS28655 19500..20003 + 504 WP_000781813.1 hypothetical protein -
CU643_RS28660 20007..21236 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
CU643_RS28665 21313..21606 + 294 WP_000134733.1 LuxR family transcriptional regulator -
CU643_RS28670 21603..21818 + 216 WP_000760375.1 EexN family lipoprotein -
CU643_RS28675 22174..22794 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
CU643_RS28680 22862..23134 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33858 33858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T89282 WP_000520549.1 NZ_CP024919:18547-18795 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T89282 NZ_CP024919:18547-18795 [Klebsiella pneumoniae]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT89282 WP_000681613.1 NZ_CP024919:18792-19139 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT89282 NZ_CP024919:18792-19139 [Klebsiella pneumoniae]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References