Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 16583..17048 Replicon plasmid pKPNH54.3
Accession NZ_CP024919
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag CU643_RS28615 Protein ID WP_001230707.1
Coordinates 16583..16834 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag CU643_RS28620 Protein ID WP_000801440.1
Coordinates 16821..17048 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS28585 11679..11912 + 234 Protein_15 hypothetical protein -
CU643_RS28590 12064..12591 - 528 WP_000577043.1 DUF2726 domain-containing protein -
CU643_RS28595 12726..13130 - 405 WP_012421203.1 hypothetical protein -
CU643_RS28600 13143..14009 - 867 WP_001353743.1 replication initiation protein -
CU643_RS28935 15424..15939 + 516 WP_001025395.1 J domain-containing protein -
CU643_RS28610 16044..16586 + 543 WP_001083906.1 hypothetical protein -
CU643_RS28615 16583..16834 + 252 WP_001230707.1 hypothetical protein Toxin
CU643_RS28620 16821..17048 + 228 WP_000801440.1 hypothetical protein Antitoxin
CU643_RS28630 17502..17801 + 300 WP_000650309.1 hypothetical protein -
CU643_RS28635 17892..18242 + 351 WP_000854259.1 hypothetical protein -
CU643_RS28640 18547..18795 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
CU643_RS28645 18792..19139 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
CU643_RS28655 19500..20003 + 504 WP_000781813.1 hypothetical protein -
CU643_RS28660 20007..21236 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
CU643_RS28665 21313..21606 + 294 WP_000134733.1 LuxR family transcriptional regulator -
CU643_RS28670 21603..21818 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33858 33858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T89281 WP_001230707.1 NZ_CP024919:16583-16834 [Klebsiella pneumoniae]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T89281 NZ_CP024919:16583-16834 [Klebsiella pneumoniae]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT89281 WP_000801440.1 NZ_CP024919:16821-17048 [Klebsiella pneumoniae]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT89281 NZ_CP024919:16821-17048 [Klebsiella pneumoniae]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References