Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 19931..20456 Replicon plasmid pKPNH54.1
Accession NZ_CP024917
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CU643_RS27240 Protein ID WP_013023785.1
Coordinates 20151..20456 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CU643_RS27235 Protein ID WP_001568025.1
Coordinates 19931..20149 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS27200 15087..15866 + 780 WP_013023780.1 hypothetical protein -
CU643_RS27205 16073..17665 + 1593 WP_015344964.1 hypothetical protein -
CU643_RS27210 17699..18826 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
CU643_RS27215 18823..19113 + 291 WP_013023783.1 hypothetical protein -
CU643_RS27235 19931..20149 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CU643_RS27240 20151..20456 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CU643_RS27245 20625..21020 + 396 WP_017899885.1 hypothetical protein -
CU643_RS27250 21047..21361 + 315 WP_053389906.1 hypothetical protein -
CU643_RS27255 21372..22388 + 1017 WP_017899884.1 hypothetical protein -
CU643_RS27260 22586..23380 + 795 WP_004197635.1 site-specific integrase -
CU643_RS27265 23812..23991 - 180 WP_006788217.1 hypothetical protein -
CU643_RS27270 24111..24737 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaTEM-1C / blaCTX-M-15 / tet(A) / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB6 - 1..126018 126018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T89276 WP_013023785.1 NZ_CP024917:20151-20456 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T89276 NZ_CP024917:20151-20456 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT89276 WP_001568025.1 NZ_CP024917:19931-20149 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT89276 NZ_CP024917:19931-20149 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References