Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1267119..1267738 Replicon chromosome
Accession NZ_CP024916
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CU643_RS06255 Protein ID WP_002892050.1
Coordinates 1267119..1267337 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CU643_RS06260 Protein ID WP_002892066.1
Coordinates 1267364..1267738 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS06225 1263162..1263425 + 264 WP_032432663.1 type B 50S ribosomal protein L31 -
CU643_RS06230 1263425..1263565 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CU643_RS06235 1263562..1264260 - 699 WP_023287311.1 GNAT family N-acetyltransferase -
CU643_RS06240 1264361..1265812 + 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
CU643_RS06245 1265787..1266257 - 471 WP_020802585.1 YlaC family protein -
CU643_RS29015 1266278..1266418 + 141 WP_004147370.1 hypothetical protein -
CU643_RS06250 1266390..1266956 - 567 WP_002892030.1 maltose O-acetyltransferase -
CU643_RS06255 1267119..1267337 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CU643_RS06260 1267364..1267738 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CU643_RS06265 1268224..1271370 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CU643_RS06270 1271393..1272586 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T89261 WP_002892050.1 NZ_CP024916:c1267337-1267119 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T89261 NZ_CP024916:c1267337-1267119 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT89261 WP_002892066.1 NZ_CP024916:c1267738-1267364 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT89261 NZ_CP024916:c1267738-1267364 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References