Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 45429..46054 Replicon chromosome
Accession NZ_CP024916
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CU643_RS00230 Protein ID WP_002882817.1
Coordinates 45671..46054 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CU643_RS00225 Protein ID WP_004150355.1
Coordinates 45429..45671 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS00200 41420..42019 + 600 WP_004151865.1 glucose-1-phosphatase -
CU643_RS00205 42013..42873 + 861 WP_004146232.1 virulence factor BrkB family protein -
CU643_RS00210 42870..43307 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
CU643_RS00215 43352..44293 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CU643_RS00220 44307..45224 - 918 WP_021466676.1 alpha/beta hydrolase -
CU643_RS00225 45429..45671 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
CU643_RS00230 45671..46054 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CU643_RS00235 46228..47157 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CU643_RS00240 47154..47789 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CU643_RS00245 47786..48688 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T89257 WP_002882817.1 NZ_CP024916:45671-46054 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T89257 NZ_CP024916:45671-46054 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT89257 WP_004150355.1 NZ_CP024916:45429-45671 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT89257 NZ_CP024916:45429-45671 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References