Detailed information of TA system TA089256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 280114..280640
• Replicon: plasmid pOSUKPC4
• Accession: NZ_CP024910
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain OSUKPC4_L

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CUN65_RS25855
• Protein ID: WP_000323025.1
• Coordinates: 280114..280401 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CUN65_RS25860
• Protein ID: WP_000534858.1
• Coordinates: 280401..280640 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUN65_RS26555	275684..275860	-	177	WP_001371930.1	hypothetical protein	-
CUN65_RS25825	276371..277315	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
CUN65_RS25830	277411..278013	+	603	WP_012695474.1	hypothetical protein	-
CUN65_RS25835	278073..278423	+	351	WP_000743059.1	hypothetical protein	-
CUN65_RS25840	278470..278673	+	204	WP_001015183.1	hypothetical protein	-
CUN65_RS25845	278955..279275	+	321	WP_000332796.1	hypothetical protein	-
CUN65_RS25850	279884..280042	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
CUN65_RS25855	280114..280401	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CUN65_RS25860	280401..280640	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CUN65_RS25870	280903..281826	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
CUN65_RS25875	282026..282598	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
CUN65_RS25880	283074..284312	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / dfrA21 / blaOXA-129 / ant(3'')-Ia / qacE / sul1 / tet(B) / formA / mcr-9 / blaKPC-4	-	1..351806	351806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T89256 WP_000323025.1 NZ_CP024910:c280401-280114 [Enterobacter hormaechei subsp. xiangfangensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T89256 NZ_CP024910:c280401-280114 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT89256 WP_000534858.1 NZ_CP024910:c280640-280401 [Enterobacter hormaechei subsp. xiangfangensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT89256 NZ_CP024910:c280640-280401 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8