Detailed information of TA system TA089253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4544983..4545590
• Replicon: chromosome
• Accession: NZ_CP024908
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain OSUKPC4_L

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: CUN65_RS22675
• Protein ID: WP_057979982.1
• Coordinates: 4545405..4545590 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUN65_RS22670
• Protein ID: WP_023315016.1
• Coordinates: 4544983..4545390 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUN65_RS22655	4541726..4542979	+	1254	WP_015572749.1	valine--pyruvate transaminase	-
CUN65_RS22660	4542987..4543442	-	456	WP_023323869.1	4Fe-4S dicluster domain-containing protein	-
CUN65_RS22665	4543903..4544979	+	1077	WP_023323870.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
CUN65_RS22670	4544983..4545390	-	408	WP_023315016.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUN65_RS22675	4545405..4545590	-	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUN65_RS22680	4545840..4547378	-	1539	WP_006808612.1	aldehyde dehydrogenase AldB	-
CUN65_RS22685	4547545..4548429	+	885	WP_003860164.1	ROK family protein	-
CUN65_RS22690	4548433..4550271	-	1839	WP_023323871.1	selenocysteine-specific translation elongation factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T89253 WP_057979982.1 NZ_CP024908:c4545590-4545405 [Enterobacter hormaechei subsp. xiangfangensis]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T89253 NZ_CP024908:c4545590-4545405 [Enterobacter hormaechei subsp. xiangfangensis]
GTGAAAAGTGCGGATATCATTACTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTTACCGTCCCACATCCTAAAAAGGACATTAAACCAGGCACTCTCGCACAAATCT
GGCGACAGGCGGGAATTAAACACTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15205.20 Da
• Isoelectric Point: 4.5036

>AT89253 WP_023315016.1 NZ_CP024908:c4545390-4544983 [Enterobacter hormaechei subsp. xiangfangensis]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEMLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLPR

Nucleotide

• Length: 408 bp

>AT89253 NZ_CP024908:c4545390-4544983 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGTGGTTTCTTTCCTGACGTTCCGGGGTGTTTTTT
TGCGGGGGATTCACTGGATGATGCTTTTCAGGATGCACGTGATGCATTAACTGCCCATTTTGAAGCGTTGTTTGAAATGG
ATGAAATGTTGCCACTTCCGGGTATGGTTGAAGCGCATCTGGCAAGTCATCCGGAGGATTTCATTGGCGGACAATGGTTG
CTGGTGGATATCAATATGAAACAGTTTGAAGGCAGAGTAGAGCGCATCAATATTACGATACCTCGCCGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCACAATTTACTAACCGAAGTGCTTTTCTTGCAGAGGCAGCACGGCGTGTACTGC
CCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0