Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3559449..3560069 Replicon chromosome
Accession NZ_CP024908
Organism Enterobacter hormaechei subsp. xiangfangensis strain OSUKPC4_L

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag CUN65_RS17855 Protein ID WP_015571250.1
Coordinates 3559851..3560069 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag CUN65_RS17850 Protein ID WP_006809850.1
Coordinates 3559449..3559823 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUN65_RS17840 3554576..3555769 + 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CUN65_RS17845 3555792..3558938 + 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit -
CUN65_RS17850 3559449..3559823 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
CUN65_RS17855 3559851..3560069 + 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
CUN65_RS17860 3560278..3560829 + 552 WP_022650496.1 maltose O-acetyltransferase -
CUN65_RS17865 3560946..3561413 + 468 WP_022650495.1 hypothetical protein -
CUN65_RS17870 3561385..3562845 - 1461 WP_032608667.1 PLP-dependent aminotransferase family protein -
CUN65_RS17875 3562947..3563657 + 711 WP_022650493.1 GNAT family N-acetyltransferase -
CUN65_RS17880 3563654..3563794 - 141 WP_032608666.1 type B 50S ribosomal protein L36 -
CUN65_RS17885 3563797..3564057 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T89251 WP_015571250.1 NZ_CP024908:3559851-3560069 [Enterobacter hormaechei subsp. xiangfangensis]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T89251 NZ_CP024908:3559851-3560069 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT89251 WP_006809850.1 NZ_CP024908:3559449-3559823 [Enterobacter hormaechei subsp. xiangfangensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT89251 NZ_CP024908:3559449-3559823 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References