Detailed information of TA system TA089239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3698793..3699382
• Replicon: chromosome
• Accession: NZ_CP024905
• Organism: Paraburkholderia caledonica strain PHRS4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CUJ87_RS16600
• Protein ID: WP_114197757.1
• Coordinates: 3698793..3698975 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A4V2NED6
• Locus tag: CUJ87_RS16605
• Protein ID: WP_027776967.1
• Coordinates: 3698972..3699382 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ87_RS16570	3693879..3694610	-	732	WP_027776974.1	flagellar hook assembly protein FlgD	-
CUJ87_RS16575	3694621..3695046	-	426	WP_027776973.1	flagellar basal body rod protein FlgC	-
CUJ87_RS16580	3695149..3695640	-	492	WP_027776972.1	flagellar basal body rod protein FlgB	-
CUJ87_RS16585	3695857..3697413	+	1557	WP_027776971.1	flagellar basal body P-ring formation protein FlgA	-
CUJ87_RS16590	3697567..3697920	+	354	WP_027776970.1	flagellar biosynthesis anti-sigma factor FlgM	-
CUJ87_RS16595	3698015..3698461	+	447	WP_027776969.1	flagellar protein FlgN	-
CUJ87_RS16600	3698793..3698975	+	183	WP_114197757.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUJ87_RS16605	3698972..3699382	+	411	WP_027776967.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUJ87_RS16610	3699455..3700189	-	735	WP_020070741.1	RNA polymerase sigma factor FliA	-
CUJ87_RS16615	3700218..3701087	-	870	WP_027776966.1	flagellar biosynthesis protein FlhG	-
CUJ87_RS16620	3701080..3703062	-	1983	WP_114197759.1	flagellar biosynthesis protein FlhF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-129)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6652.87 Da
• Isoelectric Point: 11.7159

>T89239 WP_114197757.1 NZ_CP024905:3698793-3698975 [Paraburkholderia caledonica]
MYSAEVVKLVLANGWRLVRITGSHHHFRHAFKAGLVTIPHPRKDLPAGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T89239 NZ_CP024905:3698793-3698975 [Paraburkholderia caledonica]
ATGTACAGTGCCGAAGTCGTCAAACTGGTTCTGGCGAACGGCTGGCGGCTCGTCCGCATAACGGGCAGCCACCATCATTT
TCGGCACGCATTCAAAGCCGGGCTCGTGACCATCCCCCATCCCAGGAAGGATCTGCCGGCCGGCACGCTGAACAGCATCT
TGAAACAGGCAGGGCTGAAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14831.75 Da
• Isoelectric Point: 4.5591

>AT89239 WP_027776967.1 NZ_CP024905:3698972-3699382 [Paraburkholderia caledonica]
MKNLIFPIAIEAGDAQHAFGVVVPDIPGCHSAGDSLEEAYANAKEAIEAHLDTLLDEGLPIPERLTLDEHRRNPDYAGFT
WGFVTTRNIPALKKAVRINISLPEALVHEIDEYAQARGMSRSAFLALAAEHEMADA

Nucleotide

• Length: 411 bp

>AT89239 NZ_CP024905:3698972-3699382 [Paraburkholderia caledonica]
ATGAAAAATCTGATCTTCCCGATCGCAATCGAAGCGGGCGACGCACAGCACGCGTTCGGCGTCGTCGTGCCGGATATTCC
GGGCTGTCACTCGGCGGGAGATTCGCTGGAAGAAGCTTACGCGAACGCAAAAGAAGCGATCGAGGCGCATCTGGATACGC
TGCTCGATGAAGGCTTGCCGATTCCCGAAAGGCTGACGCTAGACGAGCATCGGCGTAATCCTGACTACGCGGGTTTCACG
TGGGGTTTCGTGACAACCCGGAATATTCCCGCGCTGAAAAAGGCGGTACGCATCAACATTTCGTTGCCCGAAGCGCTGGT
GCATGAAATCGACGAATACGCGCAGGCGCGTGGCATGTCGCGTTCCGCTTTTCTCGCGCTCGCGGCCGAGCATGAAATGG
CGGACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A4V2NED6