Detailed information of TA system TA089229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1778634..1779236
• Replicon: chromosome
• Accession: NZ_CP024902
• Organism: Burkholderia pyrrocinia strain mHSR5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A6P2QRH5
• Locus tag: CUJ89_RS08575
• Protein ID: WP_059771351.1
• Coordinates: 1779054..1779236 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUJ89_RS08570
• Protein ID: WP_114176945.1
• Coordinates: 1778634..1779029 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ89_RS08520	1773704..1774711	+	1008	WP_114176935.1	helix-turn-helix domain-containing protein	-
CUJ89_RS38225	1774708..1774884	+	177	WP_201752289.1	hypothetical protein	-
CUJ89_RS08525	1774881..1775444	+	564	WP_114176936.1	hypothetical protein	-
CUJ89_RS08530	1775444..1775845	+	402	WP_114176937.1	hypothetical protein	-
CUJ89_RS08535	1775842..1776285	+	444	WP_114176938.1	DUF1364 family protein	-
CUJ89_RS08540	1776288..1776620	+	333	WP_114176939.1	hypothetical protein	-
CUJ89_RS08545	1776617..1776865	+	249	WP_114176940.1	hypothetical protein	-
CUJ89_RS08550	1776862..1777110	+	249	WP_114176941.1	phage portal protein	-
CUJ89_RS08555	1777148..1777546	+	399	WP_114176942.1	hypothetical protein	-
CUJ89_RS08560	1777690..1778034	+	345	WP_152036607.1	hypothetical protein	-
CUJ89_RS08565	1778207..1778482	-	276	WP_114176944.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	-
CUJ89_RS08570	1778634..1779029	-	396	WP_114176945.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUJ89_RS08575	1779054..1779236	-	183	WP_059771351.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUJ89_RS08580	1779278..1779634	+	357	WP_114176946.1	HNH endonuclease	-
CUJ89_RS08585	1779782..1780267	+	486	WP_114176947.1	phage terminase small subunit P27 family	-
CUJ89_RS08590	1780277..1781989	+	1713	WP_114176948.1	terminase large subunit	-
CUJ89_RS08595	1781986..1782171	+	186	WP_114176949.1	hypothetical protein	-
CUJ89_RS08600	1782176..1783435	+	1260	WP_114176950.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1757989..1861796	103807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6774.99 Da
• Isoelectric Point: 11.1039

>T89229 WP_059771351.1 NZ_CP024902:c1779236-1779054 [Burkholderia pyrrocinia]
MNSTELIKRIKADGWYHVHTVGSHHQFKHPTKPGKVTVPHPKKDLLIATVRSILKQAGLK

Nucleotide

• Length: 183 bp

>T89229 NZ_CP024902:c1779236-1779054 [Burkholderia pyrrocinia]
ATGAACAGCACCGAACTCATCAAGCGAATCAAGGCCGATGGCTGGTACCACGTCCATACCGTCGGGTCGCACCATCAGTT
CAAACACCCGACAAAGCCGGGCAAGGTAACGGTGCCACACCCGAAGAAAGACCTGCTGATCGCTACGGTGCGCAGCATCC
TGAAACAAGCCGGCCTGAAATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14329.19 Da
• Isoelectric Point: 4.9028

>AT89229 WP_114176945.1 NZ_CP024902:c1779029-1778634 [Burkholderia pyrrocinia]
MLYPLYVHVGDAKHAHGVTFPDFPGCHAAADTWEDLPAAVQEAAEAHFYDDDGPVPAPSPLESLARNPEYEGGVWMIFDI
DLSKINSKAVRLNISLPERLVQQIDAVARARKLSRSAFLALAAEHEMAVHA

Nucleotide

• Length: 396 bp

>AT89229 NZ_CP024902:c1779029-1778634 [Burkholderia pyrrocinia]
ATGCTCTATCCACTCTATGTCCACGTTGGAGACGCCAAGCATGCTCACGGCGTCACCTTTCCCGACTTCCCTGGCTGTCA
CGCGGCAGCCGATACCTGGGAAGATCTTCCCGCTGCCGTACAGGAAGCGGCCGAGGCGCATTTCTACGATGACGATGGCC
CGGTGCCAGCTCCGTCGCCGCTGGAATCGCTGGCGCGCAATCCCGAATACGAAGGCGGCGTCTGGATGATCTTCGATATT
GACCTGTCGAAGATCAACTCCAAGGCAGTTCGCCTTAATATCAGCCTGCCCGAACGGCTCGTGCAGCAAATCGATGCGGT
CGCCCGCGCACGTAAGCTGTCACGCTCCGCATTCCTCGCGCTCGCTGCCGAGCACGAAATGGCTGTGCACGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6P2QRH5