Detailed information of TA system TA089222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5019667..5020110
• Replicon: chromosome
• Accession: NZ_CP024901
• Organism: Photorhabdus laumondii subsp. laumondii strain TT01

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: PluTT01m_RS22020
• Protein ID: WP_011148391.1
• Coordinates: 5019886..5020110 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7MZJ1
• Locus tag: PluTT01m_RS22015
• Protein ID: WP_011148390.1
• Coordinates: 5019667..5019885 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluTT01m_RS21990	5015425..5016414	-	990	WP_011148385.1	glycosyltransferase	-
PluTT01m_RS21995	5016794..5017990	+	1197	WP_011148386.1	aspartyl protease family protein	-
PluTT01m_RS22000	5018160..5018771	-	612	WP_011148387.1	hypothetical protein	-
PluTT01m_RS22005	5018905..5019141	-	237	WP_011148388.1	helix-turn-helix domain-containing protein	-
PluTT01m_RS22010	5019291..5019500	+	210	WP_011148389.1	helix-turn-helix domain-containing protein	-
PluTT01m_RS22015	5019667..5019885	+	219	WP_011148390.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
PluTT01m_RS22020	5019886..5020110	+	225	WP_011148391.1	CcdB family protein	Toxin
PluTT01m_RS22025	5020056..5021045	-	990	WP_011148392.1	AraC family transcriptional regulator	-
PluTT01m_RS22030	5021160..5021801	+	642	WP_011148393.1	HD domain-containing protein	-
PluTT01m_RS22035	5022037..5022273	-	237	WP_011148394.1	helix-turn-helix domain-containing protein	-
PluTT01m_RS22040	5022625..5023290	-	666	WP_109792001.1	GntR family transcriptional regulator	-
PluTT01m_RS22045	5023857..5024585	+	729	WP_011148396.1	DUF969 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-54)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8681.12 Da
• Isoelectric Point: 8.2844

>T89222 WP_011148391.1 NZ_CP024901:5019886-5020110 [Photorhabdus laumondii subsp. laumondii]
MQFVVYQYKRSESKYSMFVDVQRDIIETPERRMAIPLVEAHHFSSKVSRHLFPTSGSQFSSFVPLCCVCMNIFL

Nucleotide

• Length: 225 bp

>T89222 NZ_CP024901:5019886-5020110 [Photorhabdus laumondii subsp. laumondii]
ATGCAATTTGTTGTTTATCAATACAAACGGAGCGAAAGCAAATACAGTATGTTTGTTGATGTACAACGTGACATCATTGA
AACACCAGAAAGGCGTATGGCTATTCCTTTAGTTGAAGCGCATCATTTTTCAAGCAAGGTGAGTCGTCATCTATTTCCCA
CGTCTGGCTCTCAGTTTTCTTCATTTGTGCCGCTCTGCTGCGTCTGTATGAATATTTTTCTGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8379.39 Da
• Isoelectric Point: 8.5552

>AT89222 WP_011148390.1 NZ_CP024901:5019667-5019885 [Photorhabdus laumondii subsp. laumondii]
MKHRVNVTFDKENYLILNSAGVNISGFVNKAMTREAQRIKAESWKADNRKGLEEIAQFIEQYGSFADENRNW

Nucleotide

• Length: 219 bp

>AT89222 NZ_CP024901:5019667-5019885 [Photorhabdus laumondii subsp. laumondii]
ATGAAACATAGAGTTAACGTAACTTTTGATAAAGAAAATTATCTGATCCTGAATTCAGCCGGTGTAAATATTTCAGGTTT
TGTTAATAAAGCCATGACCAGAGAAGCTCAACGAATCAAAGCTGAAAGTTGGAAAGCAGATAATCGCAAAGGATTGGAAG
AAATTGCTCAATTTATTGAACAATATGGCTCATTTGCTGATGAAAACAGGAACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6L9JTU9